PXD011719

PXD011719 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomic selectivity profiling of PIKK and PI3K kinase inhibitors
Description	"Here, we report the development of a novel version of Kinobeads that extends kinome coverage to PIKK and PI3K kinases. This is achieved by inclusion of two affinity probes derived from the clinical PI3K/MTOR inhibitors Omipalisib and BGT226. The new affinity matrix was used to profile 13 clinical and pre-clinical PIKK/PI3K inhibitors. The large discrepancies between the PI3K affinity values obtained and reported results from recombinant assays led us to perform a phosphoproteomic experiment showing that the chemoproteomic assay is the better approximation of PI3K inhibitor action in-vivo."
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:50:33.014.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Reinecke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-15 23:31:52	ID requested
1	2019-03-29 04:00:28	announced
⏵ 2	2024-10-22 04:50:33	announced	2024-10-22: Updated project metadata.

Reinecke M, Ruprecht B, Poser S, Wiechmann S, Wilhelm M, Heinzlmeir S, Kuster B, M, é, dard G, Chemoproteomic Selectivity Profiling of PIKK and PI3K Kinase Inhibitors. ACS Chem Biol, 14(4):655-664(2019) [pubmed]

10.1021/acschembio.8b01020;

curator keyword: Technical, Biological

submitter keyword: chemoproteomics","Kinobeads, drug profiling

Bernhard Kuster
contact affiliation	"Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany"
contact email	kuster@tum.de
lab head
Maria Reinecke
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany
contact email	maria.reinecke@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011719

PRIDE project URI

• PRIDE
  1. PXD011719
     1. Label: PRIDE project
     2. Name: Chemoproteomic selectivity profiling of PIKK and PI3K kinase inhibitors