PXD011718

PXD011718 is an original dataset announced via ProteomeXchange.

Title	Integrative analysis of transcriptomics and proteomics of Longissimus dorsi muscle to identify proteins underlying intramuscular fat content in pigs
Description	The Laiwu pig is famous for its excessively higher level of IMF content, however, the exact regulatory mechanism is still unknown. The intramuscular fat content (IMF) is an economically important traits in pigs, which is controlled by multiple genes and biological pathways. In the present study, we performed an integrated transcriptome-assisted label-free quantitative proteomic analysis of longissimus dorsi (LD) muscle in Laiwu pigs at the fastest IMF deposition stage. A total of 5074 unique proteins were identified and 191 were differentially abundant proteins (DAPs) (> 1.2-fold cutoff, p < 0.05), which were hierarchically clustered in the LD muscle over two developmental stages from 120 d to 240 d. Proteins participating in the regulation of lipolysis in adipocytes and AMPK signaling pathway were identified. Six proteins were analyzed using parallel reaction monitoring (PRM) to confirm the reliability of the TMT proteomic analysis. Association networks of differentially abundant proteins revealed that these DAPs were mainly involved in ribosome biosynthesis in eukaryotic species. A comparasion between transcriptomic (mRNA) and proteomic data revealed seven differentially expressed genes corresponding to DAPs. This study, for the first time, provides some data for the elucidation of the molecular mechanism of IMF deposition in pigs.
HostingRepository	PRIDE
AnnounceDate	2020-01-09
AnnouncementXML	Submission_2020-01-09_05:39:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lihuan Qin
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-15 08:26:08	ID requested
⏵ 1	2020-01-09 05:39:16	announced

Ma C, Wang W, Wang Y, Sun Y, Kang L, Zhang Q, Jiang Y, TMT-labeled quantitative proteomic analyses on the longissimus dorsi to identify the proteins underlying intramuscular fat content in pigs. J Proteomics, 213():103630(2020) [pubmed]

submitter keyword: Pig

longissimus dorsi muscle

Intramuscular fat content

Proteomics

TMT

Protein

Yunliang Jiang
contact affiliation	Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,College of Animal Science and Veterinary Medicine, Shandong Agricultural University, No.61 Daizong Street,Taian 271018,Shandong,P.R.China;
contact email	yljiang723@aliyun.com
lab head
lihuan Qin
contact affiliation	PTM Biolabs Inc.
contact email	lihuan_qin@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011718
     1. Label: PRIDE project
     2. Name: Integrative analysis of transcriptomics and proteomics of Longissimus dorsi muscle to identify proteins underlying intramuscular fat content in pigs