PXD011716

PXD011716 is an original dataset announced via ProteomeXchange.

Title	Protein and metabolite composition of Arabidopsis stress granules
Description	• Stress granules (SGs) are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Despite their importance for stress adaptation, no complete proteome composition has been reported for plant SGs. Herein, we addressed the existing gap. Importantly, we also provide evidence for metabolite sequestration within the SGs. • To isolate SGs we used Arabidopsis seedlings expressing GFP fusion of the SGs marker protein, Rbp47b, and an experimental protocol combining differential centrifugation with affinity purification. SGs isolates were analyzed using mass spectrometry-based proteomics and metabolomics. • A quarter of the identified proteins constituted known or predicted SG components. Intriguingly, the remaining proteins were enriched in key enzymes and regulators, such as cyclin dependent kinase A (CDKA), that mediate plant responses to stress. In addition to proteins, also nucleotides, amino acids and phospholipids accumulated in SGs. • Taken together, our results indicate (i) the presence of a pre-existing SG protein interaction network, (ii) an evolutionary conservation of the proteins involved in SG assembly and dynamics, (iii) an important role for SGs in moderation of stress responses by selective storage of proteins and metabolites.
HostingRepository	PRIDE
AnnounceDate	2019-01-07
AnnouncementXML	Submission_2019-01-28_02:16:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michal Gorka
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-15 05:38:13	ID requested
1	2019-01-07 08:33:58	announced
⏵ 2	2019-01-28 02:16:45	announced	Updated publication reference for PubMed record(s): 30664249.

Kosmacz M, Gorka M, Schmidt S, Luzarowski M, Moreno JC, Szlachetko J, Leniak E, Sokolowska EM, Sofroni K, Schnittger A, Skirycz A, Protein and metabolite composition of Arabidopsis stress granules. New Phytol, 222(3):1420-1433(2019) [pubmed]

curator keyword: Biological

submitter keyword: Affinity purification, Arabidopsis thaliana, CDKA1, stress granules proteome, stress granule metabolome, stress granule extraction, stress response

Aleksandra Skirycz
contact affiliation	Department Willmitzer | Small-Molecule Signaling Max-Planck-Institut für Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam
contact email	skirycz@mpimp-golm.mpg.de
lab head
Michal Gorka
contact affiliation	Max Planck Institute for Molecular Plant Physiology
contact email	gorka@mpimp-golm.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011716

PRIDE project URI

• PRIDE
  1. PXD011716
     1. Label: PRIDE project
     2. Name: Protein and metabolite composition of Arabidopsis stress granules