PXD011703

PXD011703 is an original dataset announced via ProteomeXchange.

Title	A chemical probe for Tudor domain protein SPIN1 to investigate chromatin functions
Description	Lysine and arginine methylation are amongst the most frequent modifications on unstructured histone tails and in combination with other modifications provide the basis for a combinatorial 'chromatin or histone code'. Recognition of modified histone residues is accomplished in a specific manner by 'reader' domains that recognize chromatin modifications allowing to associate with specific effector complexes mediating chromatin functions. The methyl-lysine and methyl-arginine reader domain protein SPINDLIN1 (SPIN1) belongs to a family of 5 human genes, and has been identified as a putative oncogene and transcriptional co-activator containing three Tudor domains, able to mediate chromatin binding. Here we report on the discovery of the potent and selective bivalent Tudor domain inhibitor VinSPINIn* (see Footnote), which simultaneously engages Tudor domains 1 and 2 and effectively competes with chromatin binding. Inhibitor, chemoproteomic and knockdown studies in squamous cell carcinoma suggest an un-anticipated complexity of SPIN isoform mediated interactions in regulating cellular phenotypes.
HostingRepository	PRIDE
AnnounceDate	2022-02-23
AnnouncementXML	Submission_2022-02-23_09:06:06.455.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Lewis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-15 01:14:31	ID requested
⏵ 1	2022-02-23 09:06:06	announced

Dataset with its publication pending

curator keyword: Technical, Biological

submitter keyword: Chemical Probe, affinity enrichment

Kilian Huber
contact affiliation	Structural Genomics Consortium, University of Oxford, OX3 7DQ, Oxford, UK
contact email	Kilian.Huber@sgc.ox.ac.uk
lab head
Andrew Lewis
contact affiliation	University of Oxford
contact email	andrew.lewis@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011703
     1. Label: PRIDE project
     2. Name: A chemical probe for Tudor domain protein SPIN1 to investigate chromatin functions