PXD011701 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Detailed sub-proteome analysis of a cheese-isolated Enterococcus faecalis, provide insights into probiotic value and food safety |
Description | Enterococci are normal inhabitants of the gastrointestinal tracts of humans and animals and thanks to their capability to tolerate different environmental conditions and their high rates of gene transfer, they are able to colonize various ecological niches, as food matrices. Enterococcus faecalis bacteria are defined as “border line” microorganisms. From one side they are used as food starters, bio-control agents and probiotics to improve human or animal health. From the other side, in the last 2 two decades enterococci have emerged as important nosocomial pathogens, because bearing high-level of resistance to antibiotics and several putative virulence factors. In this study the proteomic (LC-MS/MS) and the phenotypic characterization (enzymatic methods) of three strains of E. faecalis with different origin were performed in order to investigate the differences and/or similarities occurring between pathogenic and health promoting bacteria. The E. faecalis D27 isolated as cheese contaminant, E. faecalis Symbioflor 1 a probiotic strain and E. faecalis UW3114 a clinical isolate involved in urinary tract infection were the objects of the study. The comparison of cytosolic protein expression profiles of the three strains, highlighted statistically significant changes in the abundance of proteins mainly involved in specific metabolic pathways, nutrient transport, stress response and cell wall modulation. Moreover, especially in the food contaminant and the clinical isolate, several proteins with potential pathogenic implications were found. The analysis of the extracellular proteome provided interesting results concerning proteins involved in bacterial communication, such as pheromones and conjugative elements and also proteins able to interact with human components. |
HostingRepository | PRIDE |
AnnounceDate | 2019-08-30 |
AnnouncementXML | Submission_2019-08-30_02:31:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniela Zuehlke |
SpeciesList | scientific name: Enterococcus faecalis str. Symbioflor 1; NCBI TaxID: 1261557; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-14 23:45:53 | ID requested | |
⏵ 1 | 2019-08-30 02:31:52 | announced | |
Publication List
Cirrincione S, Neumann B, Z, ü, hlke D, Riedel K, Pessione E, : A Possible Approach to Assess Food Safety and Potential Probiotic Value. Front Nutr, 6():71(2019) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: foodborne bacteria, mass spectrometry, virulence, antibiotic resistance, host interaction |
Contact List
Kathrin Riedel |
contact affiliation | Department of Microbial Physiology and Molecular Biology, Institute of Microbiology, University of Greifswald |
contact email | riedela@uni-greifswald.de |
lab head | |
Daniela Zuehlke |
contact affiliation | Ernst Moritz Arndt University Greifswald, Institute of Microbiology |
contact email | zuehlke@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD011701 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011701
- Label: PRIDE project
- Name: Detailed sub-proteome analysis of a cheese-isolated Enterococcus faecalis, provide insights into probiotic value and food safety