PXD011697

PXD011697 is an original dataset announced via ProteomeXchange.

Title	Global proteomic profiling of Salmonella infection by a giant phage
Description	The 240-kb Salmonella phage SPN3US genome encodes 264 gene products, many of which are functionally uncharacterized. We have previously used mass spectrometry to define the proteomes of wild-type and mutant forms of the SPN3US virion. In this study we sought to determine if this technique was suitable for the characterization of the SPN3US proteome during liquid infection. Mass spectrometry of SPN3US-infected cells identified 232 SPN3US and 1994 Salmonella proteins. SPN3US proteins with related functions, such as proteins with roles in DNA replication, transcription and virion formation, were coordinately expressed in a temporal manner. Mass spectral counts showed the four most abundant SPN3US proteins to be the major capsid, two head ejection proteins, and the functionally unassigned protein, gp22. This high abundance of gp22 in infected bacteria contrasted with its absence from mature virions, suggesting it might be the scaffold protein, an essential head morphogenesis protein yet to be identified in giant phages. These studies demonstrate the power of mass spectral analyses for facilitating the acquisition of new knowledge into the molecular events of viral infection.
HostingRepository	PRIDE
AnnounceDate	2018-11-21
AnnouncementXML	Submission_2019-05-27_02:08:46.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011697
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Julie Thomas
SpeciesList	scientific name: Salmonella phage SPN3US; NCBI TaxID: 1090134;
ModificationList	Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu; Pyro-carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-14 03:52:38	ID requested
1	2018-11-21 15:33:20	announced
⏵ 2	2019-05-27 02:08:47	announced	Updated publication reference for PubMed record(s): 30541839.

Weintraub ST, Mohd Redzuan NH, Barton MK, Md Amin NA, Desmond MI, Adams LE, Ali B, Pardo S, Molleur D, Wu W, Newcomb WW, Osier MV, Black LW, Steven AC, Thomas JA, Infection by a Giant Phage. J Virol, 93(5):(2019) [pubmed]

submitter keyword: Salmonella, phage SPN3US, infection

Julie Thomas
contact affiliation	Rochester Institute of Technology
contact email	jatsbi@rit.edu
lab head
Julie Thomas
contact affiliation	Rochester Institute of Technology
contact email	jatsbi@rit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD011697

PRIDE project URI

• PRIDE
  1. PXD011697
     1. Label: PRIDE project
     2. Name: Global proteomic profiling of Salmonella infection by a giant phage