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PXD011697

PXD011697 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal proteomic profiling of Salmonella infection by a giant phage
DescriptionThe 240-kb Salmonella phage SPN3US genome encodes 264 gene products, many of which are functionally uncharacterized. We have previously used mass spectrometry to define the proteomes of wild-type and mutant forms of the SPN3US virion. In this study we sought to determine if this technique was suitable for the characterization of the SPN3US proteome during liquid infection. Mass spectrometry of SPN3US-infected cells identified 232 SPN3US and 1994 Salmonella proteins. SPN3US proteins with related functions, such as proteins with roles in DNA replication, transcription and virion formation, were coordinately expressed in a temporal manner. Mass spectral counts showed the four most abundant SPN3US proteins to be the major capsid, two head ejection proteins, and the functionally unassigned protein, gp22. This high abundance of gp22 in infected bacteria contrasted with its absence from mature virions, suggesting it might be the scaffold protein, an essential head morphogenesis protein yet to be identified in giant phages. These studies demonstrate the power of mass spectral analyses for facilitating the acquisition of new knowledge into the molecular events of viral infection.
HostingRepositoryPRIDE
AnnounceDate2018-11-21
AnnouncementXMLSubmission_2019-05-27_02:08:46.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD011697
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJulie Thomas
SpeciesList scientific name: Salmonella phage SPN3US; NCBI TaxID: 1090134;
ModificationListOxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu; Pyro-carbamidomethyl
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-11-14 03:52:38ID requested
12018-11-21 15:33:20announced
22019-05-27 02:08:47announcedUpdated publication reference for PubMed record(s): 30541839.
Publication List
Weintraub ST, Mohd Redzuan NH, Barton MK, Md Amin NA, Desmond MI, Adams LE, Ali B, Pardo S, Molleur D, Wu W, Newcomb WW, Osier MV, Black LW, Steven AC, Thomas JA, Infection by a Giant Phage. J Virol, 93(5):(2019) [pubmed]
Keyword List
submitter keyword: Salmonella, phage SPN3US, infection
Contact List
Julie Thomas
contact affiliationRochester Institute of Technology
contact emailjatsbi@rit.edu
lab head
Julie Thomas
contact affiliationRochester Institute of Technology
contact emailjatsbi@rit.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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