PXD011683

PXD011683 is an original dataset announced via ProteomeXchange.

Title	The development of minimal invasive therapy of non-Hodgkin's lymphoma based on optical tweezers technology.
Description	Cell adhesion is of fundamental importance in the cell communication, signaling and motility, and its dysfunction occurs prevalently during cancer progression. The knowledge of the molecular and cellular processes involved in abnormalities in cancer cells adhesion has dramatically increased and it has been focused mainly on cellular adhesion molecules (CAMs) and tumor microenvironment. During the last decade, several methods for measuring and studying cell adhesion properties were also developed, including optical tweezers technology. We have developed a non-invasive method employing optical tweezers technology in order to differentiate normal B-cells and non-Hodgkin’s lymphoma cells derived from clinical samples. Our approach bases on nascent adhesion formation between B-cell and mesenchymal stromal cell. In this method, a single B-cell is trapped and optically seeded on mesenchymal stromal cell and maintained direct contact until stable connection between cells is formed in time-scale. After we characterized the adhesive properties of the panel of clinical samples, we performed the deep proteomics investigations on cellular adhesion proteins using liquid chromatography- tandem mass spectrometry (LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_08:27:44.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacek Wisniewski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-13 06:52:42	ID requested
⏵ 1	2021-09-08 08:27:45	announced

Du, ś, -Szachniewicz K, Rymkiewicz G, Agrawal AK, Ko, ł, odziej P, Wi, ś, niewski JR, Large-Scale Proteomic Analysis of Follicular Lymphoma Reveals Extensive Remodeling of Cell Adhesion Pathway and Identifies Hub Proteins Related to the Lymphomagenesis. Cancers (Basel), 13(4):(2021) [pubmed]

curator keyword: Technical, Biomedical

submitter keyword: optical tweezers, cell-to-cell adhesion, non-Hodgkin’s lymphoma, cellular adhesion molecules (CAMs), LC-MS/MS.

Jacek R Wisniewski
contact affiliation	Max Planck Institute of Biochemistry. Martinsried, Germany
contact email	jwisniew@biochem.mpg.de
lab head
Jacek Wisniewski
contact affiliation	Proteomics
contact email	jwisniew@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD011683

PRIDE project URI

• PRIDE
  1. PXD011683
     1. Label: PRIDE project
     2. Name: The development of minimal invasive therapy of non-Hodgkin's lymphoma based on optical tweezers technology.