PXD011677

PXD011677 is an original dataset announced via ProteomeXchange.

Title	Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation
Description	Universal proteomics sample preparation is challenging due to the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for non-specific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings presents a practical, sensitive and cost-effective proteomics sample preparation method.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:50:38.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanveer Batth
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-13 02:01:17	ID requested
1	2019-03-05 03:38:20	announced
2	2019-05-06 09:03:03	announced	Updated publication reference for PubMed record(s): 30833379.
⏵ 3	2024-10-22 04:50:39	announced	2024-10-22: Updated project metadata.

Batth TS, Tollenaere MX, R, ü, ther P, Gonzalez-Franquesa A, Prabhakar BS, Bekker-Jensen S, Deshmukh AS, Olsen JV, Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation. Mol Cell Proteomics, 18(5):1027-1035(2019) [pubmed]

10.1074/mcp.tir118.001270;

curator keyword: Technical

submitter keyword: microparticles, mass spectrometry, secretomics,Proteomics, sample preparation, phosphoproteomics, magnetic beads

Jesper Velgaard Olsen
contact affiliation	The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Tanveer Batth
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
contact email	t.batth@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011677

PRIDE project URI

• PRIDE
  1. PXD011677
     1. Label: PRIDE project
     2. Name: Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation