PXD011673

PXD011673 is an original dataset announced via ProteomeXchange.

Title	Proteomics of venom from Trimeresurus malabaricus
Description	This project mainly aims to characterize the complex toxic components present in the venom of Trimeresurus malabaricus (Malabar pit viper). Since Trimeresurus malabaricus (Malabar pit viper) species are mainly inhabited to plantation crop areas, its envenomation is a serious threat to the human population thriving in these zones, especially to the plantation workers. Therefore, exploring the venom proteome of Malabar pit viper is decisive to develop and design new antivenom and therapeutics against its envenomation. As described in this study, applying various orthogonal separation strategies helped in dissecting venom constituents of Trimeresurus malabaricus and is the first comprehensive attempt in revealing the complex venom profile of Malabar pit viper through proteomics approaches incorporating multiple database searches. In order to achieve this the crude venom components were resolved on a 12% SDS page. Further each of the bands were subjected to in-gel trypsin digestion. The crude venom was also subjected to ion-exchange chromatography separation. The obtained fractions were subjected to in-solution trypsin digestion. All the digested peptides were then subjected to Q-TOF LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2020-01-24
AnnouncementXML	Submission_2020-01-29_05:23:06.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011673
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sudarslal Sadasivan Nair
SpeciesList	scientific name: Serpentes; NCBI TaxID: 8570;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	6540 Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-13 01:50:31	ID requested
1	2020-01-24 03:25:15	announced
⏵ 2	2020-01-29 05:23:07	announced	2020-01-29: Updated publication reference for PubMed record(s): 31982532.

Vanuopadath M, Shaji SK, Raveendran D, Nair BG, Nair SS, Delineating the venom toxin arsenal of Malabar pit viper (Trimeresurus malabaricus) from the Western Ghats of India and evaluating its immunological cross-reactivity and in vitro cytotoxicity. Int J Biol Macromol, 148():1029-1045(2020) [pubmed]

ProteomeXchange project tag: Toxicoproteomics (B/D-HPP)

submitter keyword: Trimeresurus malabaricus, proteomics, ion-exchange, Malabar pit viper, SDS-PAGE

Sudarslal Sadasivan Nair
contact affiliation	Associate Professor and Head-Proteomics Research, School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri Campus, Clappana P.O, Kollam - 690525 Kerala, India, Phone-0476-2803119, 2803110
contact email	sudarslal@am.amrita.edu
lab head
Sudarslal Sadasivan Nair
contact affiliation	School of Biotechnology, Amrita Vishwa Vidyapeetham
contact email	sudarslal@am.amrita.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD011673

PRIDE project URI

• PRIDE
  1. PXD011673
     1. Label: PRIDE project
     2. Name: Proteomics of venom from Trimeresurus malabaricus