PXD011671

PXD011671 is an original dataset announced via ProteomeXchange.

Title	Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803
Description	NdbA, one of the three type 2 NAD(P)H dehydrogenases (NDH-2) in Synechocystis sp. PCC 6803 (hereafter Synechocystis) was here localized to the thylakoid membrane, unique for the three NDH-2s, and investigated with respect to photosynthetic and cellular redox metabolism. For this purpose, a deletion mutant (ΔndbA) and a complementation strain overexpressing NdbA (ΔndbA::ndbA) were constructed. It is demonstrated that NdbA is expressed at very low level in the wild type (WT) Synechocystis under photoautotrophic (PA) growth whilst substantially higher expression occurs under light activated heterotrophic growth (LAHG). The absence of NdbA resulted in non-optimal growth of Synechocystis under LAHG and concomitantly enhanced the expression of photoprotection-related flavodiiron proteins and carbon acquisition-related proteins as well as various transporters. NdbA overexpression, on the other hand, promoted photosynthetic pigmentation and functionality of photosystem I under LAHG conditions while distinct photoprotective and carbon concentrating proteins were downregulated. NdbA overexpression also exerted an effect on the expression of many signaling and gene regulation proteins. It is concluded that NdbA, which accumulates in WT thylakoid membrane particularly under LAHG conditions, has a function in redox sensing and regulation, and ultimately fine-tunes the gene expression in Synechocystis cells to meet the requirements for optimal growth under LAHG conditions.
HostingRepository	PRIDE
AnnounceDate	2019-03-15
AnnouncementXML	Submission_2019-03-15_01:50:43.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011671
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dorota Muth-Pawlak
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-13 01:49:33	ID requested
⏵ 1	2019-03-15 01:50:45	announced

Huokko T, Muth-Pawlak D, Aro EM, Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803. Plant Cell Physiol, 60(6):1386-1399(2019) [pubmed]

submitter keyword: LC-MS/MS, Synechocystis, NDH-2 dehydrogenase, light–activated heterotrophic growth, thylakoid, photosynthesis, electron transport, proteomics

Eva Mari-Aro
contact affiliation	Department of Biochemistry, Molecular Plant Biology, Tykistökatu 6 A , Turku 20520, Finland
contact email	evaaro@utu.fi
lab head
Dorota Muth-Pawlak
contact affiliation	University of Turku
contact email	dokrmu@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011671
     1. Label: PRIDE project
     2. Name: Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803