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PXD011671

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-03-15
  • AnnouncementXML: Submission_2019-03-15_01:50:43.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD011671
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Dorota Muth-Pawlak
  • Title: Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803
  • Description: NdbA, one of the three type 2 NAD(P)H dehydrogenases (NDH-2) in Synechocystis sp. PCC 6803 (hereafter Synechocystis) was here localized to the thylakoid membrane, unique for the three NDH-2s, and investigated with respect to photosynthetic and cellular redox metabolism. For this purpose, a deletion mutant (ΔndbA) and a complementation strain overexpressing NdbA (ΔndbA::ndbA) were constructed. It is demonstrated that NdbA is expressed at very low level in the wild type (WT) Synechocystis under photoautotrophic (PA) growth whilst substantially higher expression occurs under light activated heterotrophic growth (LAHG). The absence of NdbA resulted in non-optimal growth of Synechocystis under LAHG and concomitantly enhanced the expression of photoprotection-related flavodiiron proteins and carbon acquisition-related proteins as well as various transporters. NdbA overexpression, on the other hand, promoted photosynthetic pigmentation and functionality of photosystem I under LAHG conditions while distinct photoprotective and carbon concentrating proteins were downregulated. NdbA overexpression also exerted an effect on the expression of many signaling and gene regulation proteins. It is concluded that NdbA, which accumulates in WT thylakoid membrane particularly under LAHG conditions, has a function in redox sensing and regulation, and ultimately fine-tunes the gene expression in Synechocystis cells to meet the requirements for optimal growth under LAHG conditions.
  • SpeciesList: scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
  • ModificationList: Oxidation; Carbamidomethyl
  • Instrument: Q Exactive HF

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-11-13 01:49:33ID requested
12019-03-15 01:50:45announced

Publication List

  1. Huokko T, Muth-Pawlak D, Aro EM, Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803. Plant Cell Physiol, ():(2019) [pubmed]

Keyword List

  1. submitter keyword: LC-MS/MS, Synechocystis, NDH-2 dehydrogenase, light–activated heterotrophic growth, thylakoid, photosynthesis, electron transport, proteomics

Contact List

    Eva Mari-Aro
    • contact affiliation: Department of Biochemistry, Molecular Plant Biology, Tykistökatu 6 A , Turku 20520, Finland
    • contact email: evaaro@utu.fi
    • lab head:
    Dorota Muth-Pawlak
    • contact affiliation: University of Turku
    • contact email: dokrmu@utu.fi
    • dataset submitter:

Full Dataset Link List

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