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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-01-11
  • AnnouncementXML: Submission_2019-01-11_02:23:40.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Stuart Cordwell
  • Title: Proteomics of Campylobacter glycosylation
  • Description: N-linked glycosylation is an essential virulence determinant in Campylobacter jejuni, the major causative agent of gastroenteritis in the developed world. Glycosylation is encoded by the pgl gene cluster which encodes for the biosynthesis and attachment of a conserved heptasaccharide glycan to proteins in the C. jejuni periplasm. Over 80 membrane-associated proteins have been identified, however the functional role played by glycan attachment is almost completely unknown. We used quantitative proteomics by label-based and targeted strategies to examine glycosylation negative C. jejuni in comparison to wild-type. These technical approaches were considered as ‘discovery’ (label-based) and ‘validation’ data sets in our subsequent analysis. Inclusion of a glycosylation restored strain enabled us to further exploit the proteomics data to exclude non-specific protein abundance changes that could be considered as off-target effects. These data have provided a reference set of changes associated with protein N-glycosylation that could subsequently be tested by phenotypic analysis to determine the role of this modification in Campylobacter biology.
  • SpeciesList: scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: 192222;
  • ModificationList: TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive; TripleTOF 6600

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-11-09 08:22:57ID requested
12019-01-11 02:23:41announced

Publication List

  1. Cain JA, Dale AL, Niewold P, Klare WP, Man L, White MY, Scott NE, Cordwell SJ, . Mol Cell Proteomics, 18(4):715-734(2019) [pubmed]

Keyword List

  1. submitter keyword: Campylobacter, proteomics, glycosylation, LC-MS/MS, data independant acquisiton, tandem mass tag

Contact List

    Stuart Cordwell
    • contact affiliation: The University of Sydney
    • contact email: stuart.cordwell@sydney.edu.au
    • lab head:
    Stuart Cordwell
    • contact affiliation: University of Sydney
    • contact email: stuart.cordwell@sydney.edu.au
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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