PXD011646

PXD011646 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Campylobacter glycosylation
Description	N-linked glycosylation is an essential virulence determinant in Campylobacter jejuni, the major causative agent of gastroenteritis in the developed world. Glycosylation is encoded by the pgl gene cluster which encodes for the biosynthesis and attachment of a conserved heptasaccharide glycan to proteins in the C. jejuni periplasm. Over 80 membrane-associated proteins have been identified, however the functional role played by glycan attachment is almost completely unknown. We used quantitative proteomics by label-based and targeted strategies to examine glycosylation negative C. jejuni in comparison to wild-type. These technical approaches were considered as ‘discovery’ (label-based) and ‘validation’ data sets in our subsequent analysis. Inclusion of a glycosylation restored strain enabled us to further exploit the proteomics data to exclude non-specific protein abundance changes that could be considered as off-target effects. These data have provided a reference set of changes associated with protein N-glycosylation that could subsequently be tested by phenotypic analysis to determine the role of this modification in Campylobacter biology.
HostingRepository	PRIDE
AnnounceDate	2019-01-11
AnnouncementXML	Submission_2019-01-11_02:23:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stuart Cordwell
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: 192222;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-09 08:22:57	ID requested
⏵ 1	2019-01-11 02:23:41	announced

Cain JA, Dale AL, Niewold P, Klare WP, Man L, White MY, Scott NE, Cordwell SJ, . Mol Cell Proteomics, 18(4):715-734(2019) [pubmed]

submitter keyword: Campylobacter, proteomics, glycosylation, LC-MS/MS, data independant acquisiton, tandem mass tag

Stuart Cordwell
contact affiliation	The University of Sydney
contact email	stuart.cordwell@sydney.edu.au
lab head
Stuart Cordwell
contact affiliation	University of Sydney
contact email	stuart.cordwell@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011646

PRIDE project URI

• PRIDE
  1. PXD011646
     1. Label: PRIDE project
     2. Name: Proteomics of Campylobacter glycosylation