PXD011640

PXD011640 is an original dataset announced via ProteomeXchange.

Title	Comparison of Proteomic Responses
Description	To facilitate antibiotic research, we have developed a compendium of proteomic responses that allows rapid classification of compounds into those with precedented and unprecedented modes of action. We gathered more than 100 proteomic response profiles of B. subtilis to treatment with antibiotics. To depict acute bacterial responses to sublethal doses of antibiotics, we used pulse-labeling of proteins that are newly synthesized after antibiotic challenge and analyze the proteome by 2D-PAGE. Subsequently, marker proteins were excised from non-radioactive gels and identified by mass spectrometry. Through compilation of response profiles, marker proteins are delineated as signatures, which are specific for inhibition of cellular processes like DNA, RNA, or protein biosynthesis, metabolic processes like fatty acid or folic acid biosynthesis, and attacks on cellular structures like the cytoplasmic membrane or the cell wall.
HostingRepository	PRIDE
AnnounceDate	2020-10-01
AnnouncementXML	Submission_2020-10-01_00:16:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sina Schäkermann
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-09 01:30:28	ID requested
⏵ 1	2020-10-01 00:16:32	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: antibiotics, Bacillus subtilis, marker proteins

Prof. Dr. Julia Bandow
contact affiliation	Ruhr University Bochum Applied Microbiology Universitaetsstrasse 150 44801 Bochum, Germany
contact email	julia.bandow@rub.de
lab head
Sina Schäkermann
contact affiliation	Ruhr-Universität Bochum
contact email	Sina.Langklotz@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD011640

PRIDE project URI

• PRIDE
  1. PXD011640
     1. Label: PRIDE project
     2. Name: Comparison of Proteomic Responses