PXD011638
PXD011638 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | In-depth analysis of HeLa cell proteome |
Description | The tryptic peptide mixture from HeLa cells was fractionated using a High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). Each peptide fraction was subjected to LC-MS/MS in triplicate using a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific) equipped with an UltiMate 3000 liquid chromatograph (Thermo Fisher Scientific). LC-MS/MS data were integrated on a Progenesis QI for proteomics software (Waters), followed by sequence database search of the MS/MS data using Mascot software (Matrix Science). The search results showed identification of the total of 6151 protein groups, filtered at 1% false discovery rate (FDR) peptide threshold. |
HostingRepository | jPOST |
AnnounceDate | 2018-11-09 |
AnnouncementXML | Submission_2022-09-18_03:37:10.629.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kouhei Nishino |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; iodoacetamide derivatized amino-terminal residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-11-08 23:36:11 | ID requested | |
1 | 2018-11-08 23:36:15 | announced | |
2 | 2021-02-04 17:33:14 | announced | 2021-02-04: Updated FTP location. |
⏵ 3 | 2022-09-18 03:37:10 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: HeLa cells, deep proteomics, peptide fractionation |
Contact List
Takao Kawakami | |
---|---|
lab head | |
Kouhei Nishino | |
contact affiliation | Medical ProteoScope Co., Ltd. |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000518/ |