PXD011629

PXD011629 is an original dataset announced via ProteomeXchange.

Title	IP-MS target protein identification
Description	To identify the target of the monoclonal antibody, Immuoprecipitation (IP) was performed to pull-down target protein from cell culture extraction, corn or zebrafish tissue lysate. The IP product was validated on silver-stained SDS-PAGE and Western blot. The target band was cut out and performed LC-MS/MS for target protein identification.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:20:37.166.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011629
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yang Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	Deamidated; Oxidation; Trp->Oxolactone; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-08 06:06:02	ID requested
⏵ 1	2024-10-07 10:20:38	announced

10.1126/sciadv.aax2271;

Wang Z, Li Y, Hou B, Pronobis MI, Wang M, Wang Y, Cheng G, Weng W, Wang Y, Tang Y, Xu X, Pan R, Lin F, Wang N, Chen Z, Wang S, Ma LZ, Li Y, Huang D, Jiang L, Wang Z, Zeng W, Zhang Y, Du X, Lin Y, Li Z, Xia Q, Geng J, Dai H, Yu Y, Zhao XD, Yuan Z, Yan J, Nie Q, Zhang X, Wang K, Chen F, Zhang Q, Zhu Y, Zheng S, Poss KD, Tao SC, Meng X, An array of 60,000 antibodies for proteome-scale antibody generation and target discovery. Sci Adv, 6(11):eaax2271(2020) [pubmed]

10.6019/PXD011629;

submitter keyword: Human, maize, zebrafish, protein identification

Sheng-Ce Tao
contact affiliation	Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.
contact email	taosc@sjtu.edu.cn
lab head
Yang Li
contact affiliation	Shanghai Jiao Tong University
contact email	more_2351@sjtu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD011629

PRIDE project URI

• PRIDE
  1. PXD011629
     1. Label: PRIDE project
     2. Name: IP-MS target protein identification