PXD011627 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics analysis of extracellular matrix remodeling during zebrafish heart regeneration |
Description | Adult zebrafish, in contrast to mammals, are able to regenerate their hearts in response to injury or experimental amputation. Our understanding of the cellular and molecular bases that underlie this process, although fragmentary, has increased significantly over the last years. However, the role of the extracellular matrix (ECM) during zebrafish heartregeneration has been comparatively rarely explored. Here, we set out to characterize theECM protein composition inadult zebrafish hearts, and whether it changed during the regenerative response. For this purpose, we first established a decellularization protocol of adult zebrafish ventricles that significantly enriched the yield of ECM proteins. We then performed proteomic analyses of decellularized control hearts and at different times of regeneration. Our results show a dynamic change in ECM protein composition, most evident at the earliest (7 dayspost-amputation) time-point analyzed. Regeneration associated withsharp increases inspecific ECM proteins, and with an overall decrease in collagens and cytoskeletal proteins. We finally tested by atomic force microscopythat the changes in ECM composition translatedto decreased ECM stiffness. Our cumulative results identify changes in the protein composition and mechanical properties of the zebrafish heart ECM during regeneration. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:51:37.063.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joan Josep Bech-Serra |
SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-08 03:35:37 | ID requested | |
1 | 2019-08-22 03:54:17 | announced | |
2 | 2019-08-26 07:55:42 | announced | Updated publication reference for PubMed record(s): 31221719. |
⏵ 3 | 2024-10-22 04:51:44 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.ra118.001193; |
Garcia-Puig A, Mosquera JL, Jim, é, nez-Delgado S, Garc, í, a-Pastor C, Jorba I, Navajas D, Canals F, Raya A, Proteomics Analysis of Extracellular Matrix Remodeling During Zebrafish Heart Regeneration. Mol Cell Proteomics, 18(9):1745-1755(2019) [pubmed] |
Keyword List
submitter keyword: proteomic analysis, heart regeneration,extracellular matrix |
Contact List
Angel Raya |
contact affiliation | Center of Regenerative Medicine in Barcelona (CMRB) 3rd Floor Hospital Duran i Reynals Avinguda de la Gran Via, 199-203 08908 Hospitalet de Llobregat (Barcelona), Spain |
contact email | araya@cmrb.eu |
lab head | |
Joan Josep Bech-Serra |
contact affiliation | Posdoctoral Investigator -Proteomics Unit Institut d'Investigació Biomèdica de Bellvitge (IDIBELL) Hospital Duran i Reynals Avinguda de la Granvia, 199 (3th Floor) 08908 L'Hospitalet de Llobregat Barcelona - Spain |
contact email | jbech@idibell.cat |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011627
- Label: PRIDE project
- Name: Proteomics analysis of extracellular matrix remodeling during zebrafish heart regeneration