PXD011622

PXD011622 is an original dataset announced via ProteomeXchange.

Title	Typing complex meningococcal vaccines to understand diversity and population structure of key vaccine antigens
Description	Vaccines to prevent capsular group B meningococcal disease have been based on proteins, as the polysaccharide capsule was deemed unsuitable due to its similarity to human tissues. Outer membrane vesicle (OMV) vaccines have been targeted at specific capsular group B epidemics in Cuba, Norway, and New Zealand, with the major immunogen determined as porin A (PorA). Subcapsular protein vaccines aim to increase breadth of coverage of meningococcal strains, by the inclusion of protein variants from many different clonal complexes (ccs). Using scalable and portable genomic techniques, it is possible to study the diversity of OMV and subcapsular proteins in relevant meningococcal populations. Shotgun proteomics identified 461 proteins in the OMVs derived from NZ98/254, a component of the Bexsero® vaccine, with a complex proteome comprised of outer membrane proteins and proteins from other cellular compartments. Amino acid sequences for 24 selected proteins were catalogued within the PubMLST Neisseria database as part of the OMV peptide Typing (OMVT) scheme. Of the 24 proteins included, there was variation in the extent of diversity and association with ccs from the most conserved peptides FbpA (NEISp0578) and putative periplasmic proteins (NEISp1063) to the most diverse TbpA (NEISp1690). There were 1752 OMVTs identified amongst 2492/3506 isolates. OMVTs were further grouped into clusters (identical at ≥18 peptide sequences), 45.3% of isolates were assigned to one of 27 OMVT clusters. Both OMVTs and clusters were strongly associated with cc, genogroup and recombinant Bexsero® antigens. The OMVT scheme represents an open-access, web-based tool for the systematic analysis of the multiple components of OMV vaccines, demonstrating that combinations of OMV proteins exist in discrete, non-overlapping combinations associated with both genogroup and Bexsero® Antigen Sequence type (BAST). This highly structured population of disease-causing meningococci is consistent with proposed effects of host immune selection and competition between allelic variants on meningococcal population structure. This has implications for future vaccine assessment, and development, especially the choice of antigen combinations. The methodology is portable and will facilitate region-specific WGS interrogation, allowing informed choices about vaccine development or implementation.
HostingRepository	PRIDE
AnnounceDate	2018-11-14
AnnouncementXML	Submission_2019-05-27_02:08:08.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011622
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jun Wheeler
SpeciesList	scientific name: Neisseria meningitidis serogroup B; NCBI TaxID: 491;
ModificationList	TMT6plex; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-08 03:30:30	ID requested
1	2018-11-14 03:42:21	announced
⏵ 2	2019-05-27 02:08:09	announced	Updated publication reference for PubMed record(s): 30687793.

Rodrigues CMC, Chan H, Vipond C, Jolley K, Harrison OB, Wheeler J, Whiting G, Feavers IM, Maiden MCJ, Typing complex meningococcal vaccines to understand diversity and population structure of key vaccine antigens. Wellcome Open Res, 3():151(2018) [pubmed]

submitter keyword: Outer membrane vesicle, Neisseria meningitidis, meningococcal, vaccines, shotgun proteomics, LC-MS/MS

Jun Wheeler
contact affiliation	National Institute for Biological Standards and Control
contact email	jun.wheeler@nibsc.org
lab head
Jun Wheeler
contact affiliation	NIBSC
contact email	jun.wheeler@nibsc.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011622
     1. Label: PRIDE project
     2. Name: Typing complex meningococcal vaccines to understand diversity and population structure of key vaccine antigens