PXD011618 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ER chemical proteomics with ER-localizable reactive molecules |
Description | The endoplasmic reticulum (ER) is an organelle that performs a variety of essential cellular functions via interactions with other organelles. Despite its important role, chemical tools for profiling the composition and dynamics of ER proteins remain very limited because of the labile nature of these proteins. Here, we developed ER-localizable reactive molecules (called ERMs) as tools for ER-focused chemical proteomics. ERMs can spontaneously localize in the ER of living cells and selectively label ER-associated proteins with a combined affinity and imaging tag, enabling tag-mediated ER protein enrichment and identification with liquid chromatography tandem-mass spectrometry (LC-MS/MS). The ERM probes could be used simultaneously with the nucleus- and mitochondria-localizable reactive molecules previously developed by our group, which enabled orthogonal organellar chemoproteomics in a single biological sample. Moreover, quantitative analysis of the dynamic changes in ER-associated proteins in response to tunicamycin-induced ER stress was performed by combining ER-specific labeling with SILAC (stable isotope labeling by amino acids in cell culture)-based quantitative MS technology. Our results demonstrated that ERM-based chemical proteomics provides a powerful tool for labeling and profiling ER-related proteins in living cells. |
HostingRepository | PRIDE |
AnnounceDate | 2018-12-04 |
AnnouncementXML | Submission_2018-12-04_03:58:18.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD011618 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alma Fujisawa |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotin-maleimide; Oxidation; Acetyl; Carbamidomethyl |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-08 00:12:09 | ID requested | |
1 | 2018-11-21 05:46:20 | announced | |
⏵ 2 | 2018-12-04 03:58:19 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: ER, chemical proteomics, HeLa cell |
Contact List
Itaru Hamachi |
contact affiliation | Department of Synthetic Chemistry & Biological Chemistry, Graduate School of Engineering, Kyoto Univ. Katsura, Kyoto 615-8510, JAPAN |
contact email | ihamachi@sbchem.kyoto-u.ac.jp |
lab head | |
Alma Fujisawa |
contact affiliation | Kyoto Univ. |
contact email | fujisawa.alma.83w@st.kyoto-u.ac.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011618
- Label: PRIDE project
- Name: ER chemical proteomics with ER-localizable reactive molecules