PXD011612

PXD011612 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of okra (Abelmoschus esculentusL.) seedlings under salt stress
Description	Background Salinization seriously threatens land use efficiency and crop yields across the world. Understanding the mechanisms plants use to protect against salt stress will help breeders develop salt-tolerant vegetable crops. Okra (Abelmoschus esculentus L) is an important vegetable crop of the mallow family, which is now cultivated in warm regions worldwide. To understand the effects of salt stress on the protein level of okra, a comparative proteomic analysis of okra seedlings grown in the presence of 0 or 300 mmol L-1 NaCl treatment was performed using an integrated approach of Tandem Mass Tag labeling and LC-MS/MS integrated approach. Results A total of 7179 proteins was identified in this study, for which quantitative information was available for 5,774 proteins. In the NaCl/control comparison group, there were 317 differentially expressed proteins (DEPs), of which 165 proteins were upregulated and 152 proteins downregulatedin the presence of NaCl. Based on the above data, we carried out a systematic bioinformatics analysis of proteins with information, including protein annotation, domain characteristics,functional classification,and pathway enrichment. Enriched gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the DEPs were most strongly associated with“response to stress” and “protein processing in endoplasmic reticulum”. Furthermore, several heat shock proteins were identified as DEPs. Conclusions This informationprovides a reference direction for further research on the okraproteome in the downstreamof the salt stress response, with our data revealing that the responses of okra to salt stress involves by various pathways.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:50:08.329.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaonan fu
SpeciesList	scientific name: Abelmoschus esculentus; NCBI TaxID: 455045;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-07 08:22:57	ID requested
1	2019-11-12 15:32:01	announced
⏵ 2	2024-10-22 04:50:16	announced	2024-10-22: Updated project metadata.

10.1186/s12864-019-5737-7;

Zhan Y, Wu Q, Chen Y, Tang M, Sun C, Sun J, Yu C, Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stress. BMC Genomics, 20(1):381(2019) [pubmed]

curator keyword: Biological

submitter keyword: TMT labeling,Differential expression protein,proteomics,salt stress,heat shock proteins,okra

Chenliang Yu
contact affiliation	Institute of Agricultural Equipment, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
contact email	210070130@zju.edu.cn
lab head
xiaonan fu
contact affiliation	zhejiang scientific technology universitiy
contact email	2016460649@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011612

PRIDE project URI

• PRIDE
  1. PXD011612
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of okra (Abelmoschus esculentusL.) seedlings under salt stress