PXD011610

PXD011610 is an original dataset announced via ProteomeXchange.

Title	Ammonium resupply-triggered response mechanism of lysine crotonylome in tea leaves (Camellia sinensis L.)
Description	Background: Lysine crotonylation (Kcr), as a novel evolutionarily conserved type of PTM, is ubiquitous and essential in cell biology. However, its functions in tea plant, an important beverage crop, are largely unknown. Our study firstly attempted to describe Kcr proteins in tea leaves under NH4+ deficiency/resupply, and provided significant insights into exploring the physiological role of Kcr in plants for N utilization. Results: We performed the global analysis of crotonylome in tea plants under NH4+ deficiency/resupply using high-resolution LC-MS/MS coupled with highly sensitive immune-antibody. A total of 2288 kcr sites on 971 proteins were identified, of which contained in 15 types of Kcr motifs. Most of Kcr proteins were located in chloroplast and cytoplasm. 120 and 151 Kcr proteins were significantly changed at 3 hours and 3days of NH4+ resupply, respectively. Bioinformatics analysis showed that differentially expressed Kcr proteins participated in diverse biological processes such as photosynthesis, carbon fixation and amino acid metabolism, suggesting Kcr plays important roles in these processes. Interestingly, a large number of enzymes were crotonylated, and the activity and Kcr level of these enzymes changed significantly after NH4+ resupply, indicating a potential function of Kcr in the regulation of enzyme activities. Moreover, the protein-protein interaction analysis revealed that the diverse interactions of identified Kcr proteins mainly involved in photosynthesis, carbon fixation, amino acid metabolism and ribosome. Conclusions: The results suggested that lysine crotonylated proteins might play regulating roles in metabolic process in tea leaves under NH4+ deficiency/resupply. The critical regulatory roles mainly involved in diverse aspects of primary metabolic processes, especially in photosynthesis, carbon fixation and amino acid metabolism. The provided data may serve as important resources for exploring the physiological, biochemical, and genetic role of lysine crotonylation in tea plants.
HostingRepository	PRIDE
AnnounceDate	2019-05-09
AnnouncementXML	Submission_2019-05-09_00:27:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lihuan Qin
SpeciesList	scientific name: Camellia sinensis; NCBI TaxID: 4442;
ModificationList	N6-crotonyl-L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-07 08:13:44	ID requested
⏵ 1	2019-05-09 00:27:17	announced

Sun J, Qiu C, Qian W, Wang Y, Sun L, Li Y, Ding Z, Ammonium triggered the response mechanism of lysine crotonylome in tea plants. BMC Genomics, 20(1):340(2019) [pubmed]

curator keyword: Biological

submitter keyword: Lysine crotonylation, Crotonylome, Camellia sinensis L., Photosynthesis, Carbon fixation, Amino acid metabolism, Enzymatic activity

Zhaotang Ding
contact affiliation	Tea Research Institute, Qingdao Agricultural University, Qingdao, Shandong 266109, China
contact email	dzttea@163.com
lab head
lihuan Qin
contact affiliation	PTM Biolabs Inc.
contact email	lihuan_qin@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011610
     1. Label: PRIDE project
     2. Name: Ammonium resupply-triggered response mechanism of lysine crotonylome in tea leaves (Camellia sinensis L.)