PXD011602

PXD011602 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS of RIBOPROTEOMES OF HEALTHY, POTATO VIRUS A- AND AGROBACTERIUM -INFECTED NICOTIANA BENTHAMIANA PLANTS
Description	Nicotiana benthamiana is an important model plant for plant-microbe interaction studies. We compared the proteomes of ribosomes purified from healthy N. benthamiana plants and plants that were infected with two plant pathogens potato virus A (PVA genus Potyvirus) and A. tumefaciens bacteria. Ribosomes were affinity purified from transgenic leaves that expressed FLAG-tagged RPL18B (RPL: ribosome protein large subunit) of A. thaliana. Control purifications were made from non-transgenic plants that were infected with PVA. Our riboproteome revealed ~6600 r-protein hits representing 424 distinct r-proteins that were members from 71 of the expected 81 r-protein families.
HostingRepository	PRIDE
AnnounceDate	2018-12-05
AnnouncementXML	Submission_2018-12-05_07:48:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katri Eskelin
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Potato virus A; NCBI TaxID: 12215; scientific name: Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter); NCBI TaxID: 358;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-07 06:04:43	ID requested
⏵ 1	2018-12-05 07:48:09	announced

Eskelin K, Varjosalo M, Ravantti J, M, ä, kinen K, Ribosome profiles and riboproteomes of healthy and Potato virus A- and Agrobacterium-infected Nicotiana benthamiana plants. Mol Plant Pathol, 20(3):392-409(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Ribosome, LC-MSMS, Nicotiana benthamiana, plant, potato virus a, Agrobacterium tumefaciens, healthy plants

Katri Eskelin
contact affiliation	Molecular and Integrative Biosciences Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Finland
contact email	katri.eskelin@helsinki.fi
lab head
Katri Eskelin
contact affiliation	University of Helsinki
contact email	katri.eskelin@helsinki.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD011602

PRIDE project URI

• PRIDE
  1. PXD011602
     1. Label: PRIDE project
     2. Name: LC-MSMS of RIBOPROTEOMES OF HEALTHY, POTATO VIRUS A- AND AGROBACTERIUM -INFECTED NICOTIANA BENTHAMIANA PLANTS