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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-12-05
  • AnnouncementXML: Submission_2018-12-05_07:48:07.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Katri Eskelin
  • Description: Nicotiana benthamiana is an important model plant for plant-microbe interaction studies. We compared the proteomes of ribosomes purified from healthy N. benthamiana plants and plants that were infected with two plant pathogens potato virus A (PVA genus Potyvirus) and A. tumefaciens bacteria. Ribosomes were affinity purified from transgenic leaves that expressed FLAG-tagged RPL18B (RPL: ribosome protein large subunit) of A. thaliana. Control purifications were made from non-transgenic plants that were infected with PVA. Our riboproteome revealed ~6600 r-protein hits representing 424 distinct r-proteins that were members from 71 of the expected 81 r-protein families.
  • SpeciesList: scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Potato virus A; NCBI TaxID: 12215; scientific name: Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter); NCBI TaxID: 358;
  • ModificationList: phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-11-07 06:04:43ID requested
12018-12-05 07:48:09announced

Publication List

  1. Eskelin K, Varjosalo M, Ravantti J, Mäkinen K, Ribosome profiles and riboproteomes of healthy and Potato virus A- and Agrobacterium-infected Nicotiana benthamiana plants. Mol Plant Pathol, 20(3):392-409(2019) [pubmed]

Keyword List

  1. curator keyword: Biomedical
  2. submitter keyword: Ribosome, LC-MSMS, Nicotiana benthamiana, plant, potato virus a, Agrobacterium tumefaciens, healthy plants

Contact List

    Katri Eskelin
    • contact affiliation: Molecular and Integrative Biosciences Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Finland
    • contact email: katri.eskelin@helsinki.fi
    • lab head:
    Katri Eskelin
    • contact affiliation: University of Helsinki
    • contact email: katri.eskelin@helsinki.fi
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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