PXD011589

PXD011589 is an original dataset announced via ProteomeXchange.

Title	Profiling tissue-specific modulation of gene expression in response to insulin signalling in Drosophila
Description	We investigated tissue-specific regulation of gene expression in a long lived Drosophila IIS mutant (dilp2-3,5) compared to its control (wDah). As in the majority of Drosophila laboratory strains, the endosymbiont Wolbachia was present; in the absence of Wolbachia, life extension through dilp2-3,5 is abrogated (Grönke et al. 2010). To control for this, we additionally included strains of the same genotypes that lacked Wolbachia (dilp2-3,5T, wDahT). We quantified gene expression concurrently on the level of the proteome and the transcriptome, in four key tissues: brain, gut, fat body, and muscle. Proteome quantification was carried out on five biological replicates per experimental group. Corresponding transcriptome quantification was carried out on three biological replicates per experimental group, and published on GEO (accession number *GSE122190*)
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:21:25.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Sehlke
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-06 01:52:02	ID requested
1	2021-04-21 08:49:04	announced
2	2021-04-22 00:18:04	announced	2021-04-22: Updated project metadata.
3	2021-04-22 21:55:31	announced	2021-04-22: Updated project metadata. 2021-04-23: Updated publication reference for PubMed record(s): 33879316.
⏵ 4	2024-10-22 05:21:29	announced	2024-10-22: Updated project metadata.

10.7554/elife.67275;

Tain LS, Sehlke R, Meilenbrock RL, Leech T, Paulitz J, Chokkalingam M, Nagaraj N, Gr, ö, nke S, Fr, ö, hlich J, Atanassov I, Mann M, Beyer A, Partridge L, . Elife, 10():(2021) [pubmed]

curator keyword: Biological

submitter keyword: drosophila tissue-specific insulin longevity lifespan aging shotgun

Linda Partridge
contact affiliation	Max Planck Institute for Biology of Ageing, Cologne, Germany
contact email	Partridge@age.mpg.de
lab head
Robert Sehlke
contact affiliation	Max Planck Institute for Biology of Ageing
contact email	robert@sehlke.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD011589

PRIDE project URI

• PRIDE
  1. PXD011589
     1. Label: PRIDE project
     2. Name: Profiling tissue-specific modulation of gene expression in response to insulin signalling in Drosophila