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PXD011589

PXD011589 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProfiling tissue-specific modulation of gene expression in response to insulin signalling in Drosophila
DescriptionWe investigated tissue-specific regulation of gene expression in a long lived Drosophila IIS mutant (dilp2-3,5) compared to its control (wDah). As in the majority of Drosophila laboratory strains, the endosymbiont Wolbachia was present; in the absence of Wolbachia, life extension through dilp2-3,5 is abrogated (Grönke et al. 2010). To control for this, we additionally included strains of the same genotypes that lacked Wolbachia (dilp2-3,5T, wDahT). We quantified gene expression concurrently on the level of the proteome and the transcriptome, in four key tissues: brain, gut, fat body, and muscle. Proteome quantification was carried out on five biological replicates per experimental group. Corresponding transcriptome quantification was carried out on three biological replicates per experimental group, and published on GEO (accession number *GSE122190*)
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:21:25.508.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRobert Sehlke
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-11-06 01:52:02ID requested
12021-04-21 08:49:04announced
22021-04-22 00:18:04announced2021-04-22: Updated project metadata.
32021-04-22 21:55:31announced2021-04-22: Updated project metadata.
2021-04-23: Updated publication reference for PubMed record(s): 33879316.
42024-10-22 05:21:29announced2024-10-22: Updated project metadata.
Publication List
10.7554/elife.67275;
Tain LS, Sehlke R, Meilenbrock RL, Leech T, Paulitz J, Chokkalingam M, Nagaraj N, Gr, ö, nke S, Fr, ö, hlich J, Atanassov I, Mann M, Beyer A, Partridge L, . Elife, 10():(2021) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: drosophila tissue-specific insulin longevity lifespan aging shotgun
Contact List
Linda Partridge
contact affiliationMax Planck Institute for Biology of Ageing, Cologne, Germany
contact emailPartridge@age.mpg.de
lab head
Robert Sehlke
contact affiliationMax Planck Institute for Biology of Ageing
contact emailrobert@sehlke.net
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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