PXD011589 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Profiling tissue-specific modulation of gene expression in response to insulin signalling in Drosophila |
Description | We investigated tissue-specific regulation of gene expression in a long lived Drosophila IIS mutant (dilp2-3,5) compared to its control (wDah). As in the majority of Drosophila laboratory strains, the endosymbiont Wolbachia was present; in the absence of Wolbachia, life extension through dilp2-3,5 is abrogated (Grönke et al. 2010). To control for this, we additionally included strains of the same genotypes that lacked Wolbachia (dilp2-3,5T, wDahT). We quantified gene expression concurrently on the level of the proteome and the transcriptome, in four key tissues: brain, gut, fat body, and muscle. Proteome quantification was carried out on five biological replicates per experimental group. Corresponding transcriptome quantification was carried out on three biological replicates per experimental group, and published on GEO (accession number *GSE122190*) |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:21:25.508.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Robert Sehlke |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-06 01:52:02 | ID requested | |
1 | 2021-04-21 08:49:04 | announced | |
2 | 2021-04-22 00:18:04 | announced | 2021-04-22: Updated project metadata. |
3 | 2021-04-22 21:55:31 | announced | 2021-04-22: Updated project metadata.
2021-04-23: Updated publication reference for PubMed record(s): 33879316. |
⏵ 4 | 2024-10-22 05:21:29 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.7554/elife.67275; |
Tain LS, Sehlke R, Meilenbrock RL, Leech T, Paulitz J, Chokkalingam M, Nagaraj N, Gr, ö, nke S, Fr, ö, hlich J, Atanassov I, Mann M, Beyer A, Partridge L, . Elife, 10():(2021) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: drosophila tissue-specific insulin longevity lifespan aging shotgun |
Contact List
Linda Partridge |
contact affiliation | Max Planck Institute for Biology of Ageing, Cologne, Germany |
contact email | Partridge@age.mpg.de |
lab head | |
Robert Sehlke |
contact affiliation | Max Planck Institute for Biology of Ageing |
contact email | robert@sehlke.net |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011589
- Label: PRIDE project
- Name: Profiling tissue-specific modulation of gene expression in response to insulin signalling in Drosophila