PXD011587

PXD011587 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of RBOHF by CIPK11 and CIPK26 in vitro
Description	RBOHF from Arabidopsis thaliana represents a multifunctional NADPH oxidase regulating biotic and abiotic stress tolerance, developmental processes and guard cell aperture. The molecular components and mechanisms determining RBOHF activity remain to be elucidated. While the Ca2+ sensor-activated kinases CIPK11 and CIPK26 constitute alternative paths for RBOHF activation, the combined activity of CIPKs and the kinase open stomata 1 (OST1) triggers complementary activation of this NADPH oxidase, which is efficiently counteracted through dephosphorylation by the phosphatase ABI1. Within RBOHF, several distinct phosphorylation sites (p-sites) in the N-terminus of RBOHF appear to contribute individually to activity regulation.
HostingRepository	PRIDE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2018-12-04_04:18:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Scholz
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-06 00:54:18	ID requested
1	2018-11-06 07:52:06	announced
⏵ 2	2018-12-04 04:18:58	announced	Updated project metadata.

Han JP, K, ö, ster P, Drerup MM, Scholz M, Li S, Edel KH, Hashimoto K, Kuchitsu K, Hippler M, Kudla J, binding. New Phytol, 221(4):1935-1949(2019) [pubmed]

curator keyword: Biological

submitter keyword: RBOHF, calcium, CIPK, phosphorylation, ABA, Arabidopsis thaliana

Michael Hippler
contact affiliation	WWU Münster IBBP
contact email	mhippler@uni-muenster.de
lab head
Martin Scholz
contact affiliation	IBBP Muenster
contact email	mscholz@uni-muenster.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011587
     1. Label: PRIDE project
     2. Name: Phosphorylation of RBOHF by CIPK11 and CIPK26 in vitro