PXD011577

PXD011577 is an original dataset announced via ProteomeXchange.

Title	lnc-EAPV binding proteins in C57BL/6J BMDMs
Description	Endogenous retroviruses (ERVs) are transposable elements that cause host genome instability and usually play deleterious roles such as tumorigenesis. Recent advances also suggest that this 'enemy within' may encode viral mimic to induce antiviral immune responses through viral sensors. Here, through whole genome RNA-seq we discovered a full-length ERV-derived long non-coding RNA (lncRNA), designated lnc-EPAV (ERV-derived lncRNA positively regulates antiviral responses), as a positive regulator of NF-κB signaling. Lnc-EPAV expression was rapidly up-regulated by viral RNA mimic or RNA viruses to facilitate the expression of RELA, an NF-κB subunit that plays a critical role in antiviral responses. In turn, RELA promoted the transcription of lnc-EPAV to form a positive feedback loop. Transcriptome analysis of lnc-EPAV-silenced macrophages, combined with gain- and loss-of-function experiments, showed that lnc-EPAV was critical for induction of type I interferon (IFN) and inflammatory cytokine expression by RNA viruses. Consistently, lnc-EPAV-deficient mice exhibited reduced expression of type I IFNs, and consequently increased viral loads and mortality following lethal RNA virus infection. Mechanistically, lnc-EPAV promoted expression of RELA by competitively binding to and displacing SFPQ, a transcriptional repressor of RELA. The binding between ERV-derived RNAs and SFPQ also existed in human cells. Altogether, our work demonstrates an alternative mechanism by which ERVs regulate antiviral immune responses.
HostingRepository	PRIDE
AnnounceDate	2018-11-07
AnnouncementXML	Submission_2018-11-07_06:16:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bin Zhou
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-05 02:30:44	ID requested
⏵ 1	2018-11-07 06:16:41	announced

Dataset with its publication pending

submitter keyword: lnc-EPAV;RBP;BMDMs

Feng Qian
contact affiliation	School of Life Sciences Fudan University
contact email	fengqian@fudan.edu.cn
lab head
Bin Zhou
contact affiliation	School of Life Sciences Fudan University
contact email	zhoubin1999fd@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011577
     1. Label: PRIDE project
     2. Name: lnc-EAPV binding proteins in C57BL/6J BMDMs