PXD011574
PXD011574 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multi-parameter optimization of two common proteomics quantification methods for quantifying low-abundance proteins |
| Description | To improve the sensitivity and accuracy of label-free quantification and tandem mass tags (TMT) labeling in quantifying low-abundance proteins, multi-parameter optimization was carried out using a complex 2-proteome artificial sample mixture for a series of steps from sample preparation to data analysis, including the desalting of peptides, peptide injection amount for LC-MS/MS, MS1 resolution, the length of LC-MS/MS gradient, AGC targets, ion accumulation time, MS2 resolution, precursor co-isolation threshold, data analysis software, statistical calculation methods and protein fold changes. Five subprojects were included for this project. |
| HostingRepository | iProX |
| AnnounceDate | 2018-11-02 |
| AnnouncementXML | Submission_2019-06-17_20:52:51.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Piliang Hao |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue; deaminated residue; TMT6plex reporter fragment |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-11-04 19:12:18 | ID requested | |
| 1 | 2018-11-04 19:14:58 | announced | |
| 2 | 2019-06-17 20:33:20 | announced | Update publication information. |
| ⏵ 3 | 2019-06-17 20:52:52 | announced | Update publication information. |
Publication List
| Zhang C, Shi Z, Han Y, Ren Y, Hao P, Multiparameter Optimization of Two Common Proteomics Quantification Methods for Quantifying Low-Abundance Proteins. J Proteome Res, 18(1):461-468(2019) [pubmed] |
Keyword List
| submitter keyword: quantitative proteomics, label-free quantification, tandem mass tags, low-abundance proteins, mass spectrometry |
Contact List
| Piliang Hao | |
|---|---|
| contact affiliation | ShanghaiTech University |
| contact email | haopl@shanghaitech.edu.cn |
| lab head | |
| Piliang Hao | |
| contact affiliation | ShanghaiTech University |
| contact email | haopl@shanghaitech.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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