Update publication information. Many studies have shown that space environments can affect bacteria to varying degrees and cause a range of mutations. However, so far, there have been no relevant studies on the effects of long-term space flight (>1 month) on bacteria and the effects of different space residence time on bacteria. Therefore, we utilized the Staphylococcus warneri isolated from the Shenzhou-10 spacecraft that had experienced a space flight to carry into space again, in order to study the influence of the long flight formed by two space flights on the proteomic level.