PXD011565

PXD011565 is an original dataset announced via ProteomeXchange.

Title	TACO LEAF, a putative transcriptional regulator in Arabidopsis, is a target of the protein kinase CK2
Description	During the growth of multicellular organisms, spatial and temporal patterns of gene expression are strictly regulated to ensure that developmental programs are invoked at the appropriate stage. In this work, we describe a putative transcriptional regulator in Arabidopsis, TACO LEAF (TCO), whose overexpression results in the ectopic activation of reproductive genes during vegetative growth. Isolated as an activation-tagged allele, tco-1D displays gene misexpression patterns and phenotypic abnormalities, such as curled leaves and early flowering, that resemble chromatin regulatory mutants. A role for TCO in this mode of transcriptional regulation is further supported by the subnuclear accumulation patterns of TCO protein and the genetic interactions between tco-1D and mutants of chromatin modifiers. The endogenous expression pattern of TCO and the gene misregulation displayed by tco loss-of-function mutants suggest that this factor is involved in seed development. We also demonstrate that specific serine residues of TCO protein are targeted by the ubiquitous kinase CK2. Collectively, these results identify TCO as a novel regulator of gene expression whose activity is likely influenced by phosphorylation, as is the case with many chromatin regulators
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:36.143.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danielle Swaney
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-02 02:23:39	ID requested
1	2019-01-07 02:06:48	announced
⏵ 2	2024-10-22 04:39:43	announced	2024-10-22: Updated project metadata.

10.3390/ijms20010099;

Weinman LM, Running KLD, Carey NS, Stevenson EJ, Swaney DL, Chow BY, Krogan NJ, Krogan NT, , Is a Target of the Protein Kinase CK2. Int J Mol Sci, 20(1):(2018) [pubmed]

curator keyword: Biological

submitter keyword: phosphoryation,Arabidopsis, CK2, LC-MS/MS

Nevan Krogan
contact affiliation	Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
contact email	danielle.swaney@ucsf.edu
lab head
Danielle Swaney
contact affiliation	UCSF
contact email	daniswan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011565

PRIDE project URI

• PRIDE
  1. PXD011565
     1. Label: PRIDE project
     2. Name: TACO LEAF, a putative transcriptional regulator in Arabidopsis, is a target of the protein kinase CK2