PXD011552

PXD011552 is an original dataset announced via ProteomeXchange.

Title	SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity
Description	Arabidopsis lines expressing 35S::Strep-SUMO1H89R in Col-0 background were generated since the H89R SUMO1 variant simplifies MS/MS detection of SUMO conjugated lysines after trypsinization (Miller et al., 2010). Transgenic seedlings were treated with water or 250nM flg22 for 30 minutes and total protein extracts including membrane fractions were affinity purified with Pierce™ Streptavidin Magnetic Beads. LC-MS analysis of eluting proteins allowed identification of candidate SUMO-conjugated proteins in response to flg22 treatment.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_15:22:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adrian Brown
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-01 02:58:52	ID requested
⏵ 1	2019-11-12 15:22:01	announced

Orosa B, Yates G, Verma V, Srivastava AK, Srivastava M, Campanaro A, De Vega D, Fernandes A, Zhang C, Lee J, Bennett MJ, Sadanandom A, SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity. Nat Commun, 9(1):5185(2018) [pubmed]

submitter keyword: Arabidopsis thaliana, seedlings, SUMO, FLS2, LC-MS/MS, TripleTOF 6600

Ari Sadanandom
contact affiliation	Department of Biosciences, Durham University Durham, UK
contact email	ari.sadanandom@durham.ac.uk
lab head
Adrian Brown
contact affiliation	Durham University
contact email	a.p.brown@durham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011552

PRIDE project URI

• PRIDE
  1. PXD011552
     1. Label: PRIDE project
     2. Name: SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity