PXD011546

PXD011546 is an original dataset announced via ProteomeXchange.

Title	Neutrophil extracellular traps in the central nervous system: a novel therapeutic target in pneumococcal meningitis.
Description	Neutrophils are crucial mediators of host defense and are recruited to the central nervous system in large numbers during acute bacterial meningitis caused by S. pneumoniae. Neutrophils can release neutrophil extracellular traps (NETs) during infections to trap and kill bacteria. Intact NETs are fibrous structures and mainly consist of decondensed DNA and neutrophil-derived antimicrobial proteins. We report the extensive presence of NETs in the cerebrospinal fluid of patients with pneumococcal meningitis, and absence of NETs in other forms of meningitis caused by viruses, Borrelia and subarachnoid hemorrhage. In a rat model of meningitis, a clinical strain of pneumococci induced NET formation in the cerebrospinal fluid. Importantly, disrupting NETs using DNase I significantly reduced bacterial load, demonstrating that NETs contribute to the pathogenesis of pneumococcal meningitis in vivo. Targeting NETs using DNase may represent a novel indication for an already approved drug as a non-antibiotic therapeutic option to treat acute pneumococcal meningitis.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_15:21:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christofer Karlsson
SpeciesList	scientific name: Streptococcus pneumoniae TIGR4; NCBI TaxID: 170187; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-01 02:02:28	ID requested
⏵ 1	2019-11-12 15:21:07	announced

Mohanty T, Fisher J, Bakochi A, Neumann A, Cardoso JFP, Karlsson CAQ, Pavan C, Lundgaard I, Nilson B, Reinstrup P, Bonnevier J, Cederberg D, Malmstr, ö, m J, Bentzer P, Linder A, Neutrophil extracellular traps in the central nervous system hinder bacterial clearance during pneumococcal meningitis. Nat Commun, 10(1):1667(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: NETs Neutrophil Pneumococci Meningitis DDA SWATH

Johan Malmström
contact affiliation	Lund University, Division of Infection Medicine, Department of Clinical Sciences, Lund, Sweden
contact email	johan.malmstrom@med.lu.se
lab head
Christofer Karlsson
contact affiliation	Lund University
contact email	Christofer.Karlsson@med.lu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011546
     1. Label: PRIDE project
     2. Name: Neutrophil extracellular traps in the central nervous system: a novel therapeutic target in pneumococcal meningitis.