PXD011545

PXD011545 is an original dataset announced via ProteomeXchange.

Title	Systems-level Analysis of Oxygen Exposure in Zymomonas mobilis: Implications for Isoprenoid Production
Description	Zymomonas mobilis is an aerotolerant anaerobe and prolific ethanologen with attractive characteristics for industrial biofuel production. Here, we examine the effect of oxygen exposure on metabolism and gene expression in Z. mobilis by combining targeted metabolomics, mRNA sequencing, and shotgun proteomics. We found that exposure to oxygen profoundly influenced metabolism, inducing both transient metabolic bottlenecks and long-term metabolic remodeling. In particular, oxygen induced a severe but temporary metabolic bottleneck in the methyl erythritol 4-phosphate pathway for isoprenoid biosynthesis, likely caused by oxidative damage to the iron-sulfur co-factors of the final two enzymes of the pathway. This bottleneck was resolved with minimal changes in expression level of enzymes in the pathway but pronounced upregulation of enzymes related to iron-sulfur cluster maintenance and biogenesis (i.e. flavodoxin reductase and the suf operon). We also detected prominent changes in glucose utilization under aerobic conditions. Specifically, we observed increased gluconate production following exposure to oxygen, accounting for 18% of glucose uptake after 24 hours of aerobic growth. Our results suggest that under aerobic conditions, electrons from the oxidation of glucose to gluconate are delivered to the electron transport chain to minimize oxidative damage by reducing reactive oxygen species such as H2O2. This model is supported by the simultaneous upregulation of three membrane-bound dehydrogenases, cytochrome c peroxidase, and a cytochrome bd terminal oxidase.
HostingRepository	PRIDE
AnnounceDate	2019-01-18
AnnouncementXML	Submission_2019-02-27_02:16:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Hebert
SpeciesList	scientific name: Zymomonas mobilis; NCBI TaxID: 542;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-01 01:19:16	ID requested
1	2019-01-18 06:56:44	announced
⏵ 2	2019-02-27 02:16:26	announced	Updated publication reference for PubMed record(s): 30801024.

Martien JI, Hebert AS, Stevenson DM, Regner MR, Khana DB, Coon JJ, Amador-Noguez D, : Implications for Isoprenoid Production. mSystems, 4(1):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Zymomonas mobilis, Isoprenoid, Biofuels, Orbitrap Fusion Lumos

Joshua Jacques Coon
contact affiliation	Genome Center of Wisconsin,Department of Chemistry, Department of Biomolecular Chemistry, and DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI 53706, USA Morgridge Institute for Research, Madison, WI 53706, USA
contact email	jcoon@chem.wisc.edu
lab head
Alexander Hebert
contact affiliation	UW madison
contact email	ahebert@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011545
     1. Label: PRIDE project
     2. Name: Systems-level Analysis of Oxygen Exposure in Zymomonas mobilis: Implications for Isoprenoid Production