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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-01-18
  • AnnouncementXML: Submission_2019-02-27_02:16:25.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Alexander Hebert
  • Title: Systems-level Analysis of Oxygen Exposure in Zymomonas mobilis: Implications for Isoprenoid Production
  • Description: Zymomonas mobilis is an aerotolerant anaerobe and prolific ethanologen with attractive characteristics for industrial biofuel production. Here, we examine the effect of oxygen exposure on metabolism and gene expression in Z. mobilis by combining targeted metabolomics, mRNA sequencing, and shotgun proteomics. We found that exposure to oxygen profoundly influenced metabolism, inducing both transient metabolic bottlenecks and long-term metabolic remodeling. In particular, oxygen induced a severe but temporary metabolic bottleneck in the methyl erythritol 4-phosphate pathway for isoprenoid biosynthesis, likely caused by oxidative damage to the iron-sulfur co-factors of the final two enzymes of the pathway. This bottleneck was resolved with minimal changes in expression level of enzymes in the pathway but pronounced upregulation of enzymes related to iron-sulfur cluster maintenance and biogenesis (i.e. flavodoxin reductase and the suf operon). We also detected prominent changes in glucose utilization under aerobic conditions. Specifically, we observed increased gluconate production following exposure to oxygen, accounting for 18% of glucose uptake after 24 hours of aerobic growth. Our results suggest that under aerobic conditions, electrons from the oxidation of glucose to gluconate are delivered to the electron transport chain to minimize oxidative damage by reducing reactive oxygen species such as H2O2. This model is supported by the simultaneous upregulation of three membrane-bound dehydrogenases, cytochrome c peroxidase, and a cytochrome bd terminal oxidase.
  • SpeciesList: scientific name: Zymomonas mobilis; NCBI TaxID: 542;
  • ModificationList: monohydroxylated residue
  • Instrument: Orbitrap Fusion Lumos

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-11-01 01:19:16ID requested
12019-01-18 06:56:44announced
22019-02-27 02:16:26announcedUpdated publication reference for PubMed record(s): 30801024.

Publication List

  1. Martien JI, Hebert AS, Stevenson DM, Regner MR, Khana DB, Coon JJ, Amador-Noguez D, : Implications for Isoprenoid Production. mSystems, 4(1):(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Zymomonas mobilis, Isoprenoid, Biofuels, Orbitrap Fusion Lumos

Contact List

    Joshua Jacques Coon
    • contact affiliation: Genome Center of Wisconsin,Department of Chemistry, Department of Biomolecular Chemistry, and DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI 53706, USA Morgridge Institute for Research, Madison, WI 53706, USA
    • contact email: jcoon@chem.wisc.edu
    • lab head:
    Alexander Hebert
    • contact affiliation: UW madison
    • contact email: ahebert@wisc.edu
    • dataset submitter:

Full Dataset Link List

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