PXD011542

PXD011542 is an original dataset announced via ProteomeXchange.

Title	Spliceosome disassembly factors ILP1 and NTR1 promote microRNA biogenesis in Arabidopsis
Description	A battery of spliceosome-associated proteins has been identified in microRNA (miRNA) biogenesis; however, the underlying mechanisms remain elusive. The intron lariat spliceosome (ILS) complex is highly conserved among eukaryotes and its disassembly marks the end of a canonical splicing cycle. In this study, we show that two conserved disassembly factors of the ILS complex, ILP1 and NTR1, positively regulate microRNA biogenesis through facilitating transcriptional elongation in Arabidopsis. ILP1 and NTR1 form a stable complex and co-regulate alternative splicing of more than a hundred genes across the genome including the core circadian gene LHY and some pri-miRNAs. Dysfunction in either ILP1 or NTR1 result in reduced RNA polymerase II occupancy at elongated regions of MIR chromatins, without affecting MIR promoter activity, pri-miRNA decay and DCL1 processing. Our results provide insights into the molecular mechanisms of spliceosomal machineries in non-coding RNA regulation.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_15:22:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junli Wang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-31 08:37:32	ID requested
⏵ 1	2019-11-12 15:22:57	announced

Wang J, Chen S, Jiang N, Li N, Wang X, Li Z, Li X, Liu H, Li L, Yang Y, Ni T, Yu C, Ma J, Zheng B, Ren G, Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana. Nucleic Acids Res, 47(15):7886-7900(2019) [pubmed]

submitter keyword: Alternative splicing, intron lariat spliceosome, miRNA, transcription, circadian clock

Guogong Ren
contact affiliation	State Key Laboratory of Genetic Engineering & Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China
contact email	gdren@fudan.edu.cn
lab head
Junli Wang
contact affiliation	Fudan University
contact email	caoliancaohua@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011542
     1. Label: PRIDE project
     2. Name: Spliceosome disassembly factors ILP1 and NTR1 promote microRNA biogenesis in Arabidopsis