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PXD011513

PXD011513 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics-based identification of ISG15 modification sites during Listeria monocytogenes infection
DescriptionISG15 is an interferon-stimulated, ubiquitin-like protein, with anti-viral and anti-bacterial activity, however its precise mechanism of action during viral or bacterial infection remains elusive. We previously found that ISG15 restricts Listeria infection both in vitro and in vivo and identified ISGylated proteins that could be responsible for the protective effect. Here, we endeavored to map the in vivo ISGylome following Listeria infection to mechanistically elucidate the function of ISG15 in host defense. To do so we combined a genetic approach employing a murine model of deregulated ISGylation with quantitative proteomics of immune-enriched endogenous ISG15 modification sites. In this way, we mapped 930 ISG15 sites in 438 proteins. In addition, we also quantified protein level changes in the host proteome following infection. Gene ontology analysis revealed that ISGylated proteins were mostly enriched in proteins which alter cellular metabolic processes, including four upstream modulators of the catabolic and antibacterial pathway of autophagy. Structural analysis further revealed that a number of ISG15 modifications can occur at catalytic sites or dimerization interfaces of enzymes. Finally, we showed that animals and cells with deregulated ISGylation have increased infection-induced autophagy through the modification of mTOR, WIPI2, and AMBRA1. Taken together, these findings ascribe a putative role of ISGylation to temporarily reprogram organismal metabolism following infection through direct modification of a variety of enzymes in the liver.
HostingRepositoryPRIDE
AnnounceDate2019-11-28
AnnouncementXMLSubmission_2020-01-13_05:24:10.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterImpens Francis
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListubiquitinylated lysine; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-10-29 08:28:20ID requested
12019-11-28 02:42:13announced
22020-01-13 05:24:11announced2020-01-13: Updated publication reference for PubMed record(s): 31772204.
Publication List
Zhang Y, Thery F, Wu NC, Luhmann EK, Dussurget O, Foecke M, Bredow C, Jiménez-Fernández D, Leandro K, Beling A, Knobeloch KP, Impens F, Cossart P, Radoshevich L, The in vivo ISGylome links ISG15 to metabolic pathways and autophagy upon Listeria monocytogenes infection. Nat Commun, 10(1):5383(2019) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: ISG15, Ubiquitin-like modification, Listeria infection, mice
Contact List
Francis Impens
contact affiliationVIB Proteomics Core VIB-UGent Center for Medical Biotechnology UGent Department of Biomolecular Medicine
contact emailfrancis.impens@vib-ugent.be
lab head
Impens Francis
contact affiliationVIB Proteomics Expertise Center
contact emailfrancis.impens@vib-ugent.be
dataset submitter
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Dataset FTP location
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