PXD011500 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability |
Description | Piwi proteins are important for germ cell development and function in almost all animals studied thus far. These proteins are guided to specific targets, such as transposable elements, by small guide RNAs, often referred to as piRNAs, or 21U RNAs in C. elegans. In this organism, even though genetic screens have uncovered a number of potential 21U RNA biogenesis factors, little is known about how these factors interact or what they do. Based on the previously identified 21U biogenesis factor PID-1, we here define a novel protein complex, PETISCO, that is required for 21U RNA biogenesis. PETISCO contains both potential 5’-cap and 5’-phosphate RNA binding activities, suggesting involvement in 5’ end processing. We define the interaction architecture of PETISCO and reveal a second function required for embryonic development. This essential function of PETISCO is not mediated by PID-1, but by TOST-1. Vice versa, TOST-1 is not involved in 21U RNA biogenesis. Both PID-1 and TOST-1 are small, intrinsically disordered proteins that interact directly with the PETISCO protein ERH-2 (enhancer of rudimentary homolog 2) using a conserved sequence motif. Finally, our data suggest an important role for TOST-1:PETISCO in SL1 homeostasis in the early embryo. Our work describes the first molecular platform for 21U RNA production in C. elegans, and reveals that 21U RNA biogenesis is built upon a much more widely used snRNA-related pathway. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:35:20.252.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | F Butter |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-29 06:49:27 | ID requested | |
1 | 2024-04-08 07:41:03 | announced | |
⏵ 2 | 2024-10-22 06:35:21 | announced | 2024-10-22: Updated project metadata. |
Publication List
Cordeiro Rodrigues RJ, de Jesus Domingues AM, Hellmann S, Dietz S, de Albuquerque BFM, Renz C, Ulrich HD, Sarkies P, Butter F, Ketting RF, PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability. Genes Dev, 33(13-14):857-870(2019) [pubmed] |
10.1101/gad.322446.118; |
Keyword List
curator keyword: Biological |
submitter keyword: C. elegans, IP, LC-MS/MS |
Contact List
Falk Butter |
contact affiliation | Institute of Molecular Biology (IMB0 |
contact email | f.butter@imb-mainz.de |
lab head | |
F Butter |
contact affiliation | Quantitative Proteomics Institute of Molecular Biology (IMB) |
contact email | f.butter@imb-mainz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011500
- Label: PRIDE project
- Name: PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability