PXD011500

PXD011500 is an original dataset announced via ProteomeXchange.

Title	PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability
Description	Piwi proteins are important for germ cell development and function in almost all animals studied thus far. These proteins are guided to specific targets, such as transposable elements, by small guide RNAs, often referred to as piRNAs, or 21U RNAs in C. elegans. In this organism, even though genetic screens have uncovered a number of potential 21U RNA biogenesis factors, little is known about how these factors interact or what they do. Based on the previously identified 21U biogenesis factor PID-1, we here define a novel protein complex, PETISCO, that is required for 21U RNA biogenesis. PETISCO contains both potential 5’-cap and 5’-phosphate RNA binding activities, suggesting involvement in 5’ end processing. We define the interaction architecture of PETISCO and reveal a second function required for embryonic development. This essential function of PETISCO is not mediated by PID-1, but by TOST-1. Vice versa, TOST-1 is not involved in 21U RNA biogenesis. Both PID-1 and TOST-1 are small, intrinsically disordered proteins that interact directly with the PETISCO protein ERH-2 (enhancer of rudimentary homolog 2) using a conserved sequence motif. Finally, our data suggest an important role for TOST-1:PETISCO in SL1 homeostasis in the early embryo. Our work describes the first molecular platform for 21U RNA production in C. elegans, and reveals that 21U RNA biogenesis is built upon a much more widely used snRNA-related pathway.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:35:20.252.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-29 06:49:27	ID requested
1	2024-04-08 07:41:03	announced
⏵ 2	2024-10-22 06:35:21	announced	2024-10-22: Updated project metadata.

Cordeiro Rodrigues RJ, de Jesus Domingues AM, Hellmann S, Dietz S, de Albuquerque BFM, Renz C, Ulrich HD, Sarkies P, Butter F, Ketting RF, PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability. Genes Dev, 33(13-14):857-870(2019) [pubmed]

10.1101/gad.322446.118;

curator keyword: Biological

submitter keyword: C. elegans, IP, LC-MS/MS

Falk Butter
contact affiliation	Institute of Molecular Biology (IMB0
contact email	f.butter@imb-mainz.de
lab head
F Butter
contact affiliation	Quantitative Proteomics Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011500
     1. Label: PRIDE project
     2. Name: PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability