PXD011495

PXD011495 is an original dataset announced via ProteomeXchange.

Title	Localisation of outer membrane proteins in Treponema denticola by quantitative proteome analyses of outer membrane vesicles and cellular fractions
Description	The identification and localisation of outer membrane proteins (Omps) and lipoproteins in pathogenic treponemes such as T. denticola (periodontitis) and T. pallidum (syphilis) has been challenging largely due to the structural uniqueness of their outer membranes (OM), the high abundance of inner membrane (IM) lipoproteins and the low abundance of Omps. In this study, label-free quantitative proteomics using MaxQuant was applied to naturally produced outer membrane vesicles (OMVs) and cellular fractions to identify 1448 T. denticola proteins. Of these, 90 proteins were localised to the OM comprising 59 lipoproteins, 25 β-barrel proteins and six other putative OM-associated proteins. 28 lipoproteins were localised to the IM and 43 proteins were assigned to the periplasm. The signal cleavage regions of the OM and IM lipoprotein sequences were different and may reveal the signal for their differential localisation to the OM and IM. Proteins significantly enriched in OMVs included dentilisin, proteins containing leucine-rich repeats and several lipoproteins containing FGE-sulfatase domains. Blue native PAGE analysis enabled the native size of the dentilisin complex and Msp to be determined and revealed that the abundant β-barrel Omps TDE2508 and TDE1717 formed large complexes. Several Omps were identified to have homologs in T. pallidum that have not yet been identified.
HostingRepository	PRIDE
AnnounceDate	2019-02-20
AnnouncementXML	Submission_2019-02-20_02:39:07.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011495
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Veith
SpeciesList	scientific name: Treponema denticola ATCC 35405; NCBI TaxID: 243275;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-29 03:31:06	ID requested
⏵ 1	2019-02-20 02:39:08	announced

Veith PD, Glew MD, Gorasia DG, Chen D, O'Brien-Simpson NM, Reynolds EC, Localization of Outer Membrane Proteins in Treponema denticola by Quantitative Proteome Analyses of Outer Membrane Vesicles and Cellular Fractions. J Proteome Res, 18(4):1567-1581(2019) [pubmed]

curator keyword: Biological

submitter keyword: Treponema denticola, outer membrane proteins, bacterial lipoproteins, Orbitrap, outer membrane vesicles, MaxQuant, localization

Eric C Reynolds
contact affiliation	Melbourne Dental School, University of Melbourne, Victoria, Australia
contact email	eric.reynolds@unimelb.edu.au
lab head
Paul Veith
contact affiliation	Melbourne Dental School
contact email	pdv@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD011495

PRIDE project URI

• PRIDE
  1. PXD011495
     1. Label: PRIDE project
     2. Name: Localisation of outer membrane proteins in Treponema denticola by quantitative proteome analyses of outer membrane vesicles and cellular fractions