PXD011478

PXD011478 is an original dataset announced via ProteomeXchange.

Title	High-throughput identification of FLT3 wild-type and mutant kinase substrate preferences and application to design of sensitive in vitro kinase assay substrates
Description	Abnormalities in the FLT3 signaling pathway play an integral role in AML disease relapse and drug resistance. Developing new and specific FLT3 tyrosine kinase inhibitors for use in combination to induction therapy is an important step to reduce disease relapse and achieve clinical remission. To develop potent FLT3 TKI requires sensitive in vitro assay that depended on efficient FLT3 artificial substrates, which there are none reported for FLT3 WT and kinase variants. The kinase assay linked with phosphoproteomics was applied as a high throughput technique to increase the known FLT3 kinase substrates (WT, ITD and D835Y) that were used to identify the FLT3 kinase variant’s preferred kinase sequence using the KINATEST-ID substrate predictive pipeline. The identified substrate sequence was used to synthesize and validate pan-FLT3 artificial substrates to monitor in vitro kinase activity in the presence of clinically relevant FLT3 TKI.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:49:55.335.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minervo Perez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-25 08:24:15	ID requested
1	2019-01-07 02:13:35	announced
⏵ 2	2024-10-22 04:50:00	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra118.001111;

Perez M, Blankenhorn J, Murray KJ, Parker LL, Kinase Assay Substrates. Mol Cell Proteomics, 18(3):477-489(2019) [pubmed]

submitter keyword: kinase assay linked with phosphoproteomics, FLT3,Acute myeloid Leukemia, Mass spectrometry, phosphoproteomics

Laurie L. Parker
contact affiliation	Associate Professor Director of Graduate Studies BMBB Biochemistry, Molecular Biology, and Biophysics 7-140 MCB 420 Washington Avenue SE Minneapolis, MN 55455
contact email	llparker@umn.edu
lab head
Minervo Perez
contact affiliation	Purdue University
contact email	perez78@purdue.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011478

PRIDE project URI

• PRIDE
  1. PXD011478
     1. Label: PRIDE project
     2. Name: High-throughput identification of FLT3 wild-type and mutant kinase substrate preferences and application to design of sensitive in vitro kinase assay substrates