PXD011474

PXD011474 is an original dataset announced via ProteomeXchange.

Title	Deoxynivalenol and A431 epidermoid squamous carcinoma cells: proteomic profiling reveals the pathways connecting ribosomal inhibition with the loss of function of the cell membrane
Description	The possibility to combine proteomics workflows to classical experimental approaches is continuously opening new insights in all branches of biological sciences. Deoxynivalenol (vomitoxin, DON) is a secondary metabolite produced by Fusarium spp. fungi and it is one of the most recurrent mycotoxins worldwide. DON is known to inhibit protein synthesis and as such interact with the cell models in multiple and complex ways whose comprehension enormously benefit of the system toxicology approach. For the purpose of this study epidermoid squamous cell carcinoma cells A431 were incubated with DON for 24h and toxin dependent variation of the proteome profile was obtained with a LC-MS/MS approach using a reversed phase nanoLC-System (Ultimate 3000RSLC Thermo Fisher Scientific, Austria) hyphenated to a High Resolution Orbitrap Mass Spectrometer (Thermo Fisher Scientific™ Q Exactive™ Classic). DON significantly down-regulated ribosomal proteins, as well as mitochondrial respiratory chain elements and transport proteins (TOMM22; TOMM40; TOMM70A). In line with the loss of mitochondrial function, altered metabolic capability was observed, with particular impairment of lipid synthesis cascade. Effect of the mycotoxin on cell membrane was verified by confocal microscopy (morphology) and by membrane fluidity measures (biophysical properties).
HostingRepository	PRIDE
AnnounceDate	2021-05-04
AnnouncementXML	Submission_2021-05-04_00:00:19.566.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011474
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-25 05:09:02	ID requested
⏵ 1	2021-05-04 00:00:20	announced

Del Favero G, Janker L, Neuditschko B, Hohenbichler J, Kiss E, Woelflingseder L, Gerner C, Marko D, Exploring the dermotoxicity of the mycotoxin deoxynivalenol: combined morphologic and proteomic profiling of human epidermal cells reveals alteration of lipid biosynthesis machinery and membrane structural integrity relevant for skin barrier function. Arch Toxicol, 95(6):2201-2221(2021) [pubmed]

curator keyword: Biomedical

submitter keyword: Deoxynivalenol (DON, vomitoxin)

A431 cells

Phosphoproteomics

Christopher Gerner
contact affiliation	University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011474
     1. Label: PRIDE project
     2. Name: Deoxynivalenol and A431 epidermoid squamous carcinoma cells: proteomic profiling reveals the pathways connecting ribosomal inhibition with the loss of function of the cell membrane