PXD011469

PXD011469 is an original dataset announced via ProteomeXchange.

Title	Threonine dehydratase couples L-cysteine metabolism to cadmium resistance in Pseudomonas stutzeri 273 through cadmium sulfide nanoparticle biomineralization
Description	Cysteine is an amino acid containing sulfhydryl (-SH) group which is the basis of cysteine involved in sulfur metabolism and heavy metal detoxification in microorganisms. Here, we demonstrate that adding L-cysteine significantly improves the cadmium resistance and removal ability of Pseudomonas stutzeri 273. Threonine dehydratase (TSD) connects L-cysteine metabolism and cadmium resistance through CdS nanoparticle biomineralization with the ability of catalyzing L-cysteine desulfuration and H2S generation in P. stutzeri 273. Gene knockout of TSD in P. stutzeri 273 results in the decrease of L-cysteine and cadmium resistance, decline of H2S generation, and reduction of CdS biosynthesis ability.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_09:39:40.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lihuan Qin
SpeciesList	scientific name: Pseudomonas stutzeri; NCBI TaxID: 316;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-25 03:37:30	ID requested
⏵ 1	2022-03-01 09:39:41	announced

Ma N, Cai R, Sun C, Threonine dehydratase enhances bacterial cadmium resistance via driving cysteine desulfuration and biomineralization of cadmium sulfide nanocrystals. J Hazard Mater, 417():126102(2021) [pubmed]

submitter keyword: Pseudomonas stutzeri 273,Mutants,qRT-PCR

Chaomin Sun
contact affiliation	Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
contact email	sunchaomin@qdio.ac.cn
lab head
lihuan Qin
contact affiliation	PTM Biolabs Inc.
contact email	lihuan_qin@ptm-biolab.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD011469

PRIDE project URI

• PRIDE
  1. PXD011469
     1. Label: PRIDE project
     2. Name: Threonine dehydratase couples L-cysteine metabolism to cadmium resistance in Pseudomonas stutzeri 273 through cadmium sulfide nanoparticle biomineralization