PXD011467

PXD011467 is an original dataset announced via ProteomeXchange.

Title	Human POP analysis
Description	Purpose: Damage to the uterosacral ligaments is an important contributor to uterine and vaginal prolapse. The aim of this study was to identify differentially expressed proteins in the uterosacral ligaments of women with and without pelvic organ prolapse and analyze their relationships to cellular mechanisms involved in the pathogenesis of pelvic organ prolapse. Experimental Design: Uterosacral ligament connective tissue from four patients with pelvic organ prolapse and four control women underwent iTRAQ analysis followed by Ingenuity Pathway Analysis of differentially expressed proteins. Differentially expressed proteins were valideated using western blot analysis. Results: A total of 1789 unique protein sequences were identified in the uterosacral ligament connective tissues. 88 proteins had expression levels that were significantly different between prolapse and control groups (≥1.2-fold). Ingenuity pathway analysis demonstrated 14 differentially expressed proteins that were associated with "Connective Tissue Function". Among them, fibromodulin(FMOD), Collagen alpha-1 (XIV) chain(COL14A1), Calponin-1 (CNN-1), Tenascin (TNC), and Galectin-1(LGALS1 appeared most likely to play a role in the etiology of pelvic organ prolapse. Conclusions and clinical relevence: We identified at least 6 proteins not previously associated with the pathogenesis of pelvic organ prolapse with biologic functions that suggest a plausible relationship to the disorder. These results may be helpful for furthering our understanding of the pathophysiological mechanisms of pelvic organ prolapse.
HostingRepository	PRIDE
AnnounceDate	2018-12-10
AnnouncementXML	Submission_2018-12-10_05:44:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011467
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hai-Tao Pan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; iTRAQ8plex; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-25 02:08:34	ID requested
⏵ 1	2018-12-10 05:44:20	announced

Li XJ, Pan HT, Chen JJ, Fu YB, Fang M, He GH, Zhang T, Ding HG, Yu B, Cheng Y, Tan YJ, Zhao FL, Morse AN, Huang HF, Proteomics of Uterosacral Ligament Connective Tissue from Women with and without Pelvic Organ Prolapse. Proteomics Clin Appl, 13(4):e1800086(2019) [pubmed]

submitter keyword: Reproductive system, Ingenuity pathway analysis

iTRAQ analysis

pelvic organ prolapse

uterosacral ligament

Xiang-Juan Li
contact affiliation	Hangzhou Women's Hospital, Hangzhou, China
contact email	lixiangjuan2018@163.com
lab head
Hai-Tao Pan
contact affiliation	Shaoxing Women and Children's Hospital, Shaoxing, China.
contact email	panhaitao2007@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011467
     1. Label: PRIDE project
     2. Name: Human POP analysis