PXD011464

PXD011464 is an original dataset announced via ProteomeXchange.

Title	Integrative MHC-I peptidome quantitation with isobaric tagging mass spectrometry
Description	Despite the appreciation of CD8+ T cell anti-tumor immune responses towards improvement patient outcomes, the MHC-I peptides that facilitate the response are poorly described. Alternatively, whether cancer therapies alter the MHC-I peptide repertoire has not been fully assessed due to the lack of quantitative strategies. In this regard, anthracyclines and ionizing radiation both increase the infiltration of CD8+ T cells into tumors but whether they do so by altering the MHC-I peptidome repertoire is not known. To investigate this, we developed a platform for screening therapy-induced MHC-I peptides by quantitative, multiplexed measurement of MHC-I peptides with tandem mass tags (TMT). We show that both ionizing radiation and the anthracycline doxorubicin induce MHC-I peptides that are largely derived from mitotic progression and cell cycle proteins. Our approach enables further strategies to understand how small molecules and other therapies alter MHC-I peptide presentation that may be harnessed for CD8+ T cell-based immunotherapies.
HostingRepository	PRIDE
AnnounceDate	2019-03-05
AnnouncementXML	Submission_2019-03-05_03:50:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011464
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Patrick Murphy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-24 02:41:16	ID requested
⏵ 1	2019-03-05 03:50:19	announced

Murphy JP, Yu Q, Konda P, Paulo JA, Jedrychowski MP, Kowalewski DJ, Schuster H, Kim Y, Clements D, Jain A, Stevanovic S, Gygi SP, Mancias JD, Gujar S, Multiplexed Relative Quantitation with Isobaric Tagging Mass Spectrometry Reveals Class I Major Histocompatibility Complex Ligand Dynamics in Response to Doxorubicin. Anal Chem, 91(8):5106-5115(2019) [pubmed]

submitter keyword: MHC-I peptidomics, Source Proteome

Shashi Gujar
contact affiliation	Department of Pathology, Dalhousie University
contact email	shashi.gujar@dal.ca
lab head
Patrick Murphy
contact affiliation	Mass Spectrometry
contact email	patrick.murphy@dal.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011464

PRIDE project URI

• PRIDE
  1. PXD011464
     1. Label: PRIDE project
     2. Name: Integrative MHC-I peptidome quantitation with isobaric tagging mass spectrometry