PXD011459

PXD011459 is an original dataset announced via ProteomeXchange.

Title	Sequential digestion with Trypsin and Elastase in cross-linking/mass spectrometry
Description	Cross-linking/mass spectrometry has become an important approach for studying protein structures and protein-protein interactions. The amino acid composition of some protein regions impedes the detection of cross-linked residues, although it would yield invaluable information for protein modelling. Here, we report on a sequential digestion strategy with trypsin and elastase to penetrate regions with a low density of trypsin cleavage sites. We exploited intrinsic substrate recognition proper-ties of elastase to specifically target larger tryptic peptides. Our application of this protocol to the TAF4-12 complex allowed us to identify cross-links in previously inaccessible regions.
HostingRepository	PRIDE
AnnounceDate	2019-03-07
AnnouncementXML	Submission_2019-03-07_00:29:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Therese Dau
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-23 06:10:46	ID requested
⏵ 1	2019-03-07 00:29:35	announced

Dataset with its publication pending

curator keyword: Technical

submitter keyword: human TAF4-12 complex, yeast, cross-linking, LC-MSMS

Juri Rappsilber
contact affiliation	Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edin-burgh EH9 3BF, U.K. Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany
contact email	juri.rappsilber@ed.ac.uk
lab head
Therese Dau
contact affiliation	University of Edinburgh
contact email	therese.dau@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011459

PRIDE project URI

• PRIDE
  1. PXD011459
     1. Label: PRIDE project
     2. Name: Sequential digestion with Trypsin and Elastase in cross-linking/mass spectrometry