PXD011442

PXD011442 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of artificially infected Dermacentor reticulatus tick vector by human pathogenic bacterium Rickettsia slovaca
Description	Ticks are vectors of different pathogens causing human and animal diseases. Particularly, Rickettsia slovaca is zoonotic infectious bacterium transmitted by Dermacentor ticks, agent of tick-borne lymphadenopathy (TIBOLA), common across Europe. Current studies point to extreme complexity of bacterial induced effects in tick host. Systems biology tools, including proteomics, greatly contribute to understanding of molecular details of pathogen-tick-host interactions. Herein we compared laboratory-infected ticks with uninfected control after four weeks of incubation. Propagation of R. slovaca was confirmed by quantitative PCR. Using DNA was confirmed infection with R. slovaca. By proteomic approach, we discovered 33 differentially abundant gel spots, 23 of them accumulated upon artificial infection with R. slovaca. Modest 6.9% of tick proteome was affected. The protein localizations showing that eight proteins spots might be secreted, three cytoplasmic, two mitochondrial, six likely having multiple localizations, one cell membrane and one nucleus. We identified following proteins defensin, serpins, glycine-rich protein, heat shock protein involved in artificially infected tick vector, Dermacentor reticulatus. Discovered differentially abundant proteins should be further evaluated as targets to block the transmission of bacterial pathogen.
HostingRepository	PRIDE
AnnounceDate	2019-08-14
AnnouncementXML	Submission_2019-08-14_05:00:13.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011442
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maksym Danchenko
SpeciesList	scientific name: Dermacentor reticulatus; NCBI TaxID: 57047;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-22 05:03:08	ID requested
⏵ 1	2019-08-14 05:00:14	announced

Flores-Ramirez G, Sallay B, Danchenko M, Lakhneko O, Š, pitalsk, á E, Skultety L, Comparative proteomics of the vector Dermacentor reticulatus revealed differentially regulated proteins associated with pathogen transmission in response to laboratory infection with Rickettsia slovaca. Parasit Vectors, 12(1):318(2019) [pubmed]

submitter keyword: Dermacentor vector, TIBOLA, two-dimensional gel electrophoresis, real-time PCR, protective antigens

Eva Spitalska
contact affiliation	Department of Rickettsiology Biomedical Research Center Sloval Republic
contact email	viruspi@savba.sk
lab head
Maksym Danchenko
contact affiliation	Institute of Virology, Biomedical Research Center
contact email	maxymdan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD011442

PRIDE project URI

• PRIDE
  1. PXD011442
     1. Label: PRIDE project
     2. Name: Comparative proteomics of artificially infected Dermacentor reticulatus tick vector by human pathogenic bacterium Rickettsia slovaca