PXD011438

PXD011438 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of Jatropha curcas seedling under chilling stress
Description	As a promising energy plant for biodiesel, Jatropha curcas is a tropical and subtropical shrub. Chilling is a major abiotic stress affecting the growth and development of J. curcas. In this study, we adopt the phosphoproteomic analysis, physiological measurement, ultrastructure observation to illustrate molecular mechanisms of J. curcas seedling under chilling (4 °C) stress. After chilling for 6 h, 308 significantly changed phosphoproteins were detected in J. curcas seedling without obvious physiological injury. When obvious physiological injury can be observed after chilling for 24 h, a total of 332 phosphoproteins were examined to be significantly changed, after recovery (28 °C) for 24 h, 291 phosphoproteins were varied at the phosphorylation level. The results of Gene Ontology analysis showed that phosphoproteins were mainly responsible for cellular protein modification process, transport, cellular component organization and signal transduction at the chilling and recovery periods. On the basis of protein-protein interaction network analysis, several protein kinases, such as SnRK2 (serine threonine-protein kinase srk2), MEKK1 (mitogen-activated protein kinase kinase kinase 1), EDR1, CDPK (calcium-dependent protein kinase), EIN2 (Ethylene-insensitive protein 2), EIN4, PI4K (phosphatidylinositol 4-kinase alpha 1) and 14-3-3 were possible responsible for cross-talk between ABA, Ca2+, ethylene, phosphoinositide and 14-3-3 mediated signaling pathways. We also highlighted the phosphorylation of HOS1 (E3 ubiquitin-protein ligase HOS1), APX (Cytosolic ascorbate peroxidase-1) and PIP2 (Aquaporin pip2) played vital roles in J. curcas seedling under chilling stress, and they will be valuable in further study form the molecular breeding perspective.
HostingRepository	PRIDE
AnnounceDate	2019-01-09
AnnouncementXML	Submission_2019-01-14_04:48:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hui Liu
SpeciesList	scientific name: Jatropha curcas; NCBI TaxID: 180498;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-22 02:55:59	ID requested
1	2019-01-09 05:19:24	announced
⏵ 2	2019-01-14 04:48:17	announced	Updated publication reference for PubMed record(s): 30626061.

Liu H, Wang FF, Peng XJ, Huang JH, Shen SH, Seedling under Chilling Stress. Int J Mol Sci, 20(1):(2019) [pubmed]

submitter keyword: Jatropha curcas

Phosphoproteomics

Seedling

Chilling stress

Regulated mechanism

Shihua Shen
contact affiliation	Key laboratory of Research and Development for Resource Plant, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
contact email	shshen@ibcas.ac.cn
lab head
Hui Liu
contact affiliation	Institute of Botany, the Chinese Academy of Sciences
contact email	huiliu@ibcas.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011438
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of Jatropha curcas seedling under chilling stress