PXD011437

PXD011437 is an original dataset announced via ProteomeXchange.

Title	Improving the purification of membrane proteins from Escherichia coli C41(DE3)
Description	The overexpression and purification of membrane proteins to high purity and homogeneity is a challenging task. Over time, several strains have been developed that decrease the toxic side-effects and thus result in higher bio mass and protein yield. However, two major contaminants have been identified in membrane protein preparations from E. coli: the outer membrane porin OmpF and AcrB, which is part of a tripartite efflux pump. Both proteins crystallise from low concentrations and diverse conditions, which make them a major problem, especially in membrane protein crystallography. In this study, we present a C41(DE3)-derived expression strains that is depleted of these two proteins.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_15:28:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-22 02:55:25	ID requested
⏵ 1	2019-11-12 15:29:00	announced

Kanonenberg K, Royes J, Kedrov A, Poschmann G, Angius F, Solgadi A, Spitz O, Kleinschrodt D, St, ü, hler K, Miroux B, Schmitt L, Shaping the lipid composition of bacterial membranes for membrane protein production. Microb Cell Fact, 18(1):131(2019) [pubmed]

submitter keyword: membrane proteins, Escherichia coli, expression strain

Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory (MPL) Biologisch-Medizinisches Forschungszentrum (BMFZ) Heinrich-Heine-Universität Düsseldorf
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011437
     1. Label: PRIDE project
     2. Name: Improving the purification of membrane proteins from Escherichia coli C41(DE3)