PXD011427

PXD011427 is an original dataset announced via ProteomeXchange.

Title	The proteomic response of a marine Vibrio to low iron and desferrioxamine siderophore bioavailability
Description	Competition for limited iron resources is a key driver of microbial community structure in many regions of the surface ocean. The bacterial siderophores ferrioxamine and amphibactin have been identified in marine surface waters, suggesting that they may represent an important bacterial strategy for obtaining iron from a scarcely populated pool. We screened several strains of marine Vibrio for the presence of putative amphibactin biosynthesis gene homologues and amphibactin production. Whole cell proteomics, siderophore isolation, and isotopically labeled iron uptake experiments were performed. Here, we show that an amphibactin-producing marine bacterium, Vibrio cyclitrophicus str. 1F-53, harbors an independently regulated uptake pathway for ferrioxamines. Proteomic analyses identified upregulation of the amphibactin NRPS system and a putative amphibactin siderophore transporter in response to low iron concentrations. In addition, multiple other transporters were upregulated, however when desferrioxamine was present, amphibactin production decreased and the ferrioxamine receptor increased in abundance. Such cheating phenotypes, which appear widespread among marine amphibactin producers, highlight the strategies that contribute to the fitness of marine bacteria in the face of iron stress. These results demonstrate siderophore producer and cheater phenotypes and highlight the cellular restructuring which is involved due to competition for iron, that shapes the community structure of marine ecosystems.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:21:13.428.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011427
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew McIlvin
SpeciesList	scientific name: Vibrio cyclitrophicus 1F53; NCBI TaxID: 1136157;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-19 08:37:41	ID requested
1	2021-04-18 22:51:26	announced
⏵ 2	2024-10-22 05:21:13	announced	2024-10-22: Updated project metadata.

10.6019/PXD011427;

curator keyword: Biological

submitter keyword: iron bioavailability, Thermo Fusion Q-exactive, ferrioxamine,siderophore

Mak Saito
contact affiliation	Woods Hole Oceanographic Institution, Marine Chemistry and Geochemistry
contact email	msaito@whoi.edu
lab head
Matthew McIlvin
contact affiliation	Woods Hole Oceanographic Inst.
contact email	mmcilvin@whoi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011427
     1. Label: PRIDE project
     2. Name: The proteomic response of a marine Vibrio to low iron and desferrioxamine siderophore bioavailability