PXD011415

PXD011415 is an original dataset announced via ProteomeXchange.

Title	piRNA-guided co-transcriptional silencing coopts nuclear export factors
Description	The PIWI-interacting RNA (piRNA) pathway is a small RNA-based immune system that controls the expression of transposons and maintains genome integrity in animal germlines1,2. In Drosophila, piRNA-guided silencing is achieved, in part, via co-transcriptional repression of transposons by Piwi. This depends on Panoramix (Panx)3,4; however, precisely how an RNA binding event silences transcription remains to be determined. Here we show that Nuclear Export Factor 2 (Nxf2) and its co-factor, Nxt1, form a complex with Panx, and are required for co-transcriptional silencing of transposons in somatic and germline cells of the ovary. Tethering of Nxf2 to either RNA or DNA results in silencing of target loci and the concomitant accumulation of repressive chromatin marks. Nxf2 and Panx proteins are mutually required for proper localization and stability. We mapped the protein domains crucial for the Nxf2/Panx complex formation and show that the amino-terminal portion of Panx is sufficient to induce transcriptional silencing. Loss of Nxf2 or Panx results in nuclear accumulation of transposon transcripts, which is for some transposons Piwi-dependent.
HostingRepository	PRIDE
AnnounceDate	2019-06-20
AnnouncementXML	Submission_2019-06-28_03:30:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marzia Munafo
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-19 03:13:25	ID requested
1	2019-06-20 02:24:40	announced
⏵ 2	2019-06-28 03:30:12	announced	Updated publication reference for PubMed record(s): 31219034.

Fabry MH, Ciabrelli F, Munaf, ò M, Eastwood EL, Kneuss E, Falciatori I, Falconio FA, Hannon GJ, Czech B, piRNA-guided co-transcriptional silencing coopts nuclear export factors. Elife, 8():(2019) [pubmed]

submitter keyword: piRNA transposons piwi nuclear export

Gregory J Hannon
contact affiliation	Cancer Research UK Cambridge Institute University of Cambridge Li Ka Shing Centre Cambridge CB2 0RE United Kingdom
contact email	Greg.Hannon@cruk.cam.ac.uk
lab head
Marzia Munafo
contact affiliation	Cambridge University
contact email	marzia.munafo@cruk.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD011415

PRIDE project URI

• PRIDE
  1. PXD011415
     1. Label: PRIDE project
     2. Name: piRNA-guided co-transcriptional silencing coopts nuclear export factors