PXD011413

PXD011413 is an original dataset announced via ProteomeXchange.

Title	Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo
Description	A long-prevailing model has held that the “seed” region (nucleotides 2-8) of a microRNA is typically sufficient to mediate target recognition and repression. However, numerous recent studies, both within the context of defining miRNA/target pairs by direct physical association and by directly assessing this model in vivo in C. elegans have brought this model into question. To test the importance of miRNA 3' pairing in vivo, in a mammalian system, we engineered a mutant murine mir-146a allele in which the 5' half of the mature microRNA retains the sequence of the wild-type mir-146a but the 3ʹ half has been altered to be anti-complementary to the wild-type miR-146a sequence. Mice homozygous or hemizygous for this mutant allele are phenotypically indistinguishable from wild-type controls and do not recapitulate any of the immunopathology previously described for mir-146a-null mice. Our results strongly support the conclusion that 3ʹ pairing is dispensable in the context of the function of a key mammalian microRNA.
HostingRepository	PRIDE
AnnounceDate	2019-02-15
AnnouncementXML	Submission_2019-02-15_14:44:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bhoomi Bhatt
SpeciesList	scientific name: Olea europaea (Common olive); NCBI TaxID: 4146; scientific name: Pan troglodytes (Chimpanzee); NCBI TaxID: 9598; scientific name: Arachis hypogaea (Peanut); NCBI TaxID: 3818; scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Ricinus communis (Castor bean); NCBI TaxID: 3988; scientific name: Halobacterium salinarum; NCBI TaxID: 2242; scientific name: Brassica napus (Rape); NCBI TaxID: 3708; scientific name: Acyrthosiphon pisum (Pea aphid); NCBI TaxID: 7029; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036; scientific name: Ictalurus punctatus (Channel catfish); NCBI TaxID: 7998; scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117; scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351; scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091; scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113; scientific name: Hepatitis C virus subtype 1a; NCBI TaxID: 31646; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; scientific name: Helicobacter pylori J99 (Campylobacter pylori J99); NCBI TaxID: 85963; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Merluccius bilinearis (Silver hake); NCBI TaxID: 79698; scientific name: Brassica oleracea var. botrytis (Cauliflower); NCBI TaxID: 3715; scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827; scientific name: Pongo pygmaeus (Bornean orangutan); NCBI TaxID: 9600; scientific name: Rice(O. sativa); NCBI TaxID: 4530; scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534; scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888; scientific name: Heliocidaris erythrogramma (Sea urchin); NCBI TaxID: 7634; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Staphylococcus aureus; NCBI TaxID: 1280; scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460; scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689; scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544; scientific name: Pinus radiata (Monterey pine); NCBI TaxID: 3347; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513; scientific name: Gadus morhua (Atlantic cod); NCBI TaxID: 8049;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-19 00:52:31	ID requested
⏵ 1	2019-02-15 14:44:10	announced

Saltzman AB, Leng M, Bhatt B, Singh P, Chan DW, Dobrolecki L, Chandrasekaran H, Choi JM, Jain A, Jung SY, Lewis MT, Ellis MJ, Malovannaya A, gpGrouper: A Peptide Grouping Algorithm for Gene-Centric Inference and Quantitation of Bottom-Up Proteomics Data. Mol Cell Proteomics, 17(11):2270-2283(2018) [pubmed]

curator keyword: Biological

submitter keyword: Mir-146a, miRNA, mouse

Joel Neilson
contact affiliation	Baylor College of Medicine, Houston, Texas, 77030
contact email	neilson@bcm.edu
lab head
Bhoomi Bhatt
contact affiliation	baylor college of medicine
contact email	bbhatt@bcm.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD011413

PRIDE project URI

• PRIDE
  1. PXD011413
     1. Label: PRIDE project
     2. Name: Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo