PXD011391

PXD011391 is an original dataset announced via ProteomeXchange.

Title	The Hanks-type kinase PknB, targeting the divisome, is not the single player in the Streptococcus thermophilus protein phosphorylation process
Description	This work focus on  in the role of Ser/Thr/Tyr protein phosphorylation in Streptococcus thermophilus, the only streptococcal species used in food fermentation. During technological processes, S. thermophilus has to adapt to various nutrition and physical-chemical stresses that require rapid adjustments. In this study, we investigated a way for S. thermophilus to regulate specific pathways namely post-translational protein modifications and more specifically protein serine/threonine/tyrosine phosphorylation. We would like to assess the specific role of the only predicted Hanks-type kinase, named PknB. We performed in parallel a global shotgun proteomics and a specific phosphoproteomics analyses on both the wild type strain and its Hanks-type kinase deletion mutant. All the analysis were performed on an Orbitrap Fusion Lumos Tribrid. We showed that the S. thermophilus Ser/Thr/Tyr phosphoproteome is of the same order of magnitude than the other streptococci ones as peptides belonging to 106 proteins (410 phosphopeptides corresponding to 161 peptide sequences with different phosphosite positions) from various metabolic pathways were found phosphorylated in one bacterial growth condition. The phosphorylation occurred for 43 % on serine, 33 % on threonine and 23 % on tyrosine.  We demonstrated that the Hanks-type kinase, named PknB in S. thermophilus, targets the divisome and is only one of the players in the Ser/Thr/Tyr phosphorylation process. Consistent with these results, the pknB deletion mutant exhibits a clear phenotype with longer whimsical chains and affected division process.
HostingRepository	PRIDE
AnnounceDate	2019-07-08
AnnouncementXML	Submission_2019-07-08_02:09:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HENRY CELINE
SpeciesList	scientific name: Streptococcus thermophilus LMD-9; NCBI TaxID: 322159;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-17 07:37:56	ID requested
⏵ 1	2019-07-08 02:09:05	announced

Henry C, Haller L, Blein-Nicolas M, Zivy M, Canette A, Verbrugghe M, M, é, zange C, Boulay M, Gardan R, Samson S, Martin V, Andr, é, -Leroux G, Monnet V, Phosphoproteome. Front Microbiol, 10():1329(2019) [pubmed]

submitter keyword: Phosphoproteome, Streptococcus thermophilus, shotgun, PknB

Céline Henry
contact affiliation	PAPPSO, MICALIS, INRA
contact email	celine.henry@jouy.inra.fr
lab head
HENRY CELINE
contact affiliation	INRA
contact email	celine.henry@jouy.inra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011391
     1. Label: PRIDE project
     2. Name: The Hanks-type kinase PknB, targeting the divisome, is not the single player in the Streptococcus thermophilus protein phosphorylation process