PXD011366

PXD011366 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Large-scale Discovery of Substrates of the Human Kinome
Description	Kinase -networks are important for cellular signal transduction. Despite tremendous efforts to uncover these signaling pathways, huge numbers of uncharacterized phosphosites still remain in the human proteome. Because of the transient nature of kinase-substrate interactions in vivo, it is almost impossible to identify direct substrates. Here, we present a novel strategy for the rapid and high-throughput discovery for of kinase substrates of kinase using quantitative proteomics. Using 385 purified kinases, we identified a total of 175,574 potential direct kinase substrates of kinases. In addition, we identified novel kinase groups, such as one group containing 30 threonine-directed kinases and another containing 15 serine/threonine/tyrosine kinase sgroup. Surprisingly, we observed that the diversity of substrates for tyrosine kinases was much higher than that for serine-threonine kinases.
HostingRepository	jPOST
AnnounceDate	2019-07-22
AnnouncementXML	Submission_2022-09-18_03:24:34.557.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naoyuki Sugiyama
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; alpha-amino dimethylated residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-16 02:10:29	ID requested
1	2019-07-21 17:32:07	announced
2	2021-02-04 17:24:36	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 03:24:35	announced	2022-09-18: Updated FTP location.

Publication List

Sugiyama N, Imamura H, Ishihama Y, Large-scale Discovery of Substrates of the Human Kinome. Sci Rep, 9(1):10503(2019) [pubmed]

Sugiyama N, Imamura H, Ishihama Y, Large-scale Discovery of Substrates of the Human Kinome. Sci Rep, 9(1):10503(2019) [pubmed]

Keyword List

submitter keyword: Homo sapiens, HeLa, in vitro kinase assay

submitter keyword: Homo sapiens, HeLa, in vitro kinase assay

Contact List

Yasushi Ishihama
lab head
Naoyuki Sugiyama
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000508/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000508/