PXD011363

PXD011363 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize
Description	Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as substantially equivalent to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent.
HostingRepository	MassIVE
AnnounceDate	2018-10-16
AnnouncementXML	Submission_2018-10-16_07:09:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MA_group
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	unknown modification: The taxonomy selected was Zea mays and three enzymatic mis-cleavages were allowed. Dynamic modifications selected on the search were Oxidation/ +15.995Da (M) and Deamidated/ +0.984 (N, Q) and static modifications were Carbamidomethyl/ +57.021Da (C), TMT10plex/ 229.163Da (K), TMT10plex/ 229.163Da (Any N-terminus).
Instrument	instrument model: Orbitrap Velos-Pro mass spectrometer

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-15 09:21:06	ID requested
⏵ 1	2018-10-16 07:09:11	announced

Publication List

Mesnage R, Agapito-Tenfen SZ, Vilperte V, Renney G, Ward M, S, é, ralini GE, Nodari RO, Antoniou MN, An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process. Sci Rep, 6():37855(2016) [pubmed]

Mesnage R, Agapito-Tenfen SZ, Vilperte V, Renney G, Ward M, S, é, ralini GE, Nodari RO, Antoniou MN, An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process. Sci Rep, 6():37855(2016) [pubmed]

Keyword List

submitter keyword: maize

submitter keyword: maize

Contact List

Michael Antoniou
contact affiliation	King's College London
contact email	michael.antoniou@kcl.ac.uk
lab head
MA_group
contact affiliation	King's College London
contact email	MA_group
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083034