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PXD011344

PXD011344 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe role of early chromatin changes in X chromosome inactivation
DescriptionDuring development, transcriptional and chromatin modification changes co-occur but the order and causality of events often remain unclear. We explore the interrelationship of these processes using the paradigm of X-chromosome inactivation (XCI). We initiate XCI in female, mouse embryonic stem cells by inducing Xist expression and monitor changes in transcription and chromatin by allele-specific TT-seq and ChIP-seq respectively. An unprecedented temporal resolution enabled identification of the earliest chromatin alterations during XCI. We demonstrate that HDAC3 interacts with both NCOR1 and NCOR2 and is pre-bound on the X chromosome where it deacetylates histones to promote efficient gene silencing. We also reveal the choreography of polycomb accumulation following Xist RNA coating, with PRC1-associated H2AK119Ub preceding PRC2-associated H3K27me3. Furthermore, polycomb-associated marks accumulate initially at large, intergenic domains and then spreads into genes but only in the context of gene silencing. Our results provide the hierarchy of chromatin events during XCI and demonstrate that some chromatin changes play key roles in mediating transcriptional silencing.
HostingRepositoryPRIDE
AnnounceDate2019-01-17
AnnouncementXMLSubmission_2019-01-17_02:26:36.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuillaume Arras
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-10-12 01:11:46ID requested
12019-01-17 02:26:37announced
Publication List
Ż, ylicz JJ, Bousard A, Ž, umer K, Dossin F, Mohammad E, da Rocha ST, Schwalb B, Syx L, Dingli F, Loew D, Cramer P, Heard E, The Implication of Early Chromatin Changes in X Chromosome Inactivation. Cell, 176(1-2):182-197.e23(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: X chromosome inactivation
mouse embryonic stem cells
epigenomics
transcriptomics
histone deacetylation
Polycomb
histone acetylation
Contact List
Damarys Loew
contact affiliationLaboratoire de Spectrometrie de Masse Proteomique Institut Curie PSL Research University
contact emailDamarys.Loew@curie.fr
lab head
Guillaume Arras
contact affiliationInstitut Curie
contact emailguillaume.arras@curie.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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