PXD011343

PXD011343 is an original dataset announced via ProteomeXchange.

Title	The parallel reaction monitoring assays in heterosis of hybrid yellow catfish Huangyou-1
Description	Heterosis is a complex biological phenomenon in which hybridization exhibits superior phenotypic characteristics. The underlying molecular basis for heterosis, particularly for fishes, remains elusive. The utilization rate of fish heterosis far exceeds our understanding of it on a theoretical level. To gain a comprehensive and unbiased molecular understanding of fish heterosis, we characterized mRNA-seq, miRNA-seq, and proteomes of Pelteobagrus fulvidraco, P. vachelli, and the hybrid yellow catfish Huangyou-1 (P. fulvidraco ♀×P. vachelli ♂) livers using next-generation sequencing technologies and tandem mass tags. Our results show that the nonadditive, homoeologue expression bias and expression level dominance pattern were readily identified in transcriptional, post-transcriptional, or protein levels, providing the evidence for the widespread presence of dominant models during hybridization. At the same time, a number of miRNA-mRNA-protein pairs were found and validated by qRT-PCR and parallel reaction monitoring assays.
HostingRepository	iProX
AnnounceDate	2019-04-26
AnnouncementXML	Submission_2019-12-11_21:45:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaowu Yin
SpeciesList	scientific name: Tachysurus fulvidraco; NCBI TaxID: 1234273; scientific name: Tachysurus fulvidraco x Tachysurus vachelli; NCBI TaxID: 1610892; scientific name: Tachysurus vachellii; NCBI TaxID: 175792;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-11 20:36:41	ID requested
1	2018-10-11 20:37:19	announced
2	2019-04-25 22:49:35	announced	Update data files.
⏵ 3	2019-12-11 21:45:19	announced	2019-12-12: Update publication information.

Zhang G, Li J, Zhang J, Liang X, Zhang X, Wang T, Yin S, Integrated Analysis of Transcriptomic, miRNA and Proteomic Changes of a Novel Hybrid Yellow Catfish Uncovers Key Roles for miRNAs in Heterosis. Mol Cell Proteomics, 18(7):1437-1453(2019) [pubmed]

submitter keyword: parallel reaction monitoring, heterosis, proteome, hybrid catfish

Shaowu Yin
contact affiliation	Nanjing normal university
contact email	yinshaowu@163.com
lab head
Shaowu Yin
contact affiliation	Nanjing normal university
contact email	yinshaowu@163.com
dataset submitter

iProX dataset URI